Rat monoclonal [LO-MG 1-2] Anti-Mouse IgG1 heavy chain (FITC) (ab11588)

Overview

  • Product name
    Rat monoclonal [LO-MG 1-2] Anti-Mouse IgG1 heavy chain (FITC)
    See all IgG1 secondary antibodies
  • Host species
    Rat
  • Target species
    Mouse
  • Specificity
    ab11588 recognises murine IgG1, and does not bind to other mouse immunoglobulin classes or subclasses.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Purified murine IgG1 from BALB/c mice.

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    LO-MG 1-2
  • Myeloma
    IR983F
  • Isotype
    IgG1
  • General notes
    This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml. Use 10 µl of this concentration to label 106 cells in 100µl.

References

This product has been referenced in:
  • Baron DM  et al. Adverse effects of hemorrhagic shock resuscitation with stored blood are ameliorated by inhaled nitric oxide in lambs*. Crit Care Med 41:2492-501 (2013). Sheep . Read more (PubMed: 23887236) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and interest.

You could certainly use our advanced search engine specifically designed for secondary antibodies to find the most suitable products for your studies:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918



Since the isotype of this primary antibody is mouse IgG1, so you could select the isotype, target species, host species, format, specificity, conjugate using the drop-down menu.

We have different conjugates such as FITC, DyLight488, Chromeo 488, and here are a few suggestions:

FITC:

ab98692

ab97239

ab11588

You can find some further information about the the generation of our fluorochromes at this site:https://www.abcam.com/index.html?pageconfig=resource&rid=12948

DyLight488:

Affinity purified: ab96875, ab97015, ab96871

Pre-adsorbed: ab96879

Chromeo 488:

ab60313



If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

Thank you for your enquiry. For staining mouse tissue with mouse monoclonal antibodies, please refer to the following two articles: Fung KM, Messing A, Lee VM, Trojanowski JQ. A novel modification of the avidin-biotin complex method for immunohistochemical studies of transgenic mice with murine monoclonal antibodies. J Histochem Cytochem. 1992 Sep;40(9):1319-28. PMID: 1506669 Hierck BP, Iperen LV, Gittenberger-De Groot AC, Poelmann RE. Modified indirect immunodetection allows study of murine tissue with mouse monoclonal antibodies. J Histochem Cytochem. 1994 Nov;42(11):1499-502. PMID: 7930532 These two references were suggested by the following website: http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/MOMeasy.html This site also mentions a simpler approach, with results illustrated with a set of images: use a secondary antibody that will only recognize the heavy chain of the IgG isotype of the primary. Since each of the isotypes present in mouse is only 20-25% of the total IgG, background due to staining of endogenous IgG will be reduced, compared to using an anti-IgG that recognizes all isotypes. Antibody ab17833 against xanthine oxidase is isotype IgG1. Our only conjugated antibodies specific for IgG1 heavy chain are FITC conjugates, ab48229: https://www.abcam.com/index.html?datasheet=48229 or ab11588: https://www.abcam.com/index.html?datasheet=11588. For light microscopy instead of fluorescent detection, you could use either goat anti-mouse IgG1 ab9165, or rabbit anti-mouse IgG1 ab9172, but these are unconjugated and would need to be followed by a HRP- or AP-conjugated anti-rabbit or anti-goat IgG. I hope this helps. Please do not hesitate to contact me if you have any questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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