Rat monoclonal [LO-MG1-2] Anti-Mouse IgG1 heavy chain (Biotin) (ab11587)

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Overview

  • Product name

    Rat monoclonal [LO-MG1-2] Anti-Mouse IgG1 heavy chain (Biotin)
    See all IgG1 secondary antibodies

  • Host species

    Rat

  • Target species

    Mouse

  • Specificity

    ab11587 binds to the heavy chain of murine IgG1.

  • Tested applications

    Suitable for: ELISAmore details

  • Immunogen

    Monoclonal mouse IgG1.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified IgG prepared by affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    LO-MG1-2

  • Myeloma

    IR983F

  • Isotype

    IgG1

  • General notes

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at a concentration of 0.125 µg/ml, as detecting antibody.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab11587? Please let us know so that we can cite the reference in this datasheet.

    ab11587 has been referenced in 3 publications.

    • Bergman ML  et al. Tolerogenic insulin peptide therapy precipitates type 1 diabetes. J Exp Med 214:2153-2156 (2017). PubMed: 28536239
    • Daniel C  et al. Prevention of type 1 diabetes in mice by tolerogenic vaccination with a strong agonist insulin mimetope. J Exp Med 208:1501-10 (2011). ELISA ; Mouse . PubMed: 21690251
    • Babaya N  et al. Murine high specificity/sensitivity competitive europium insulin autoantibody assay. Diabetes Technol Ther 11:227-33 (2009). ELISA . PubMed: 19344197

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question



    Inquiry: This antibody is described as an anti-IgG1 mAb. Although IgG1 was the immunogen used and it would bind IgG1, from the information provided it is not clear whether this antibody has been tested for binding to other subclasses of IgG (e.g. IgG2a, IgG2b, IgG3) or to IgA, IgM, or IgE. Has reactivity to other Ig classes/subclasses been tested and is it specific only to IgG1? thanks Colin

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    Answer

    Thank you for contacting us.


    Our rat anti mouse IgG1 heavy chain antibody, recognizesmouse IgG1, and does not bind to other mouse immunoglobulin classes (such as IgM) or subclasses (such as IgG2a).





    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    My ELISA is not working.

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    Answer

    Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Below please find the link to the questionnaire we discussed over the phone. Once you have submitted the form to us, we will look at the protocol you used and see if there are any suggestions we can make to improve your results. If we determine that the antibody is not working as stated on the datasheet, and you have purchased it in the last 90 days, we would be happy to replace or refund the antibody for you. The questionnaire can be found at: https://www.abcam.com/index.html?section=elisa&pageconfig=technical&intAbID=13781&mode=questionaire I look forward to receiving your reply.

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