Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain (ab11566)

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Overview

  • Product name
    Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain
    See all IgG2b secondary antibodies
  • Host species
    Rat
  • Target species
    Mouse
  • Specificity
    ab11566 recognises murine IgG2b, binding to the gamma 2b heavy chain.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Polyclonal IgG from BALB/c mice

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    LO-MG2b-1
  • Myeloma
    IR983F
  • Isotype
    IgG1
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA (coating): Use at a concentration of 5 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    ab11566 has not yet been referenced specifically in any publications.

    Publishing research using ab11566? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

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    Answer

    Thank you for your enquiry.


    Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

    Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



    I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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