Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain (ab11566)

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Key features and details

  • Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain
  • Host species: Rat
  • Isotype: IgG1
  • Suitable for: ELISA

Overview

  • Product name

    Rat monoclonal [LO-MG2b-1] Anti-Mouse IgG2b heavy chain
    See all IgG2b secondary antibodies

  • Host species

    Rat

  • Target species

    Mouse

  • Specificity

    ab11566 recognises murine IgG2b, binding to the gamma 2b heavy chain.

  • Tested applications

    Suitable for: ELISAmore details

  • Immunogen

    Full length protein corresponding to Mouse IgG2b. Polyclonal IgG from BALB/c mice.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified IgG prepared by affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    LO-MG2b-1

  • Myeloma

    IR983F

  • Isotype

    IgG1

  • General notes

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Notes
  • Application notes
    ELISA (coating): Use at a concentration of 5 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab11566? Please let us know so that we can cite the reference in this datasheet.

    ab11566 has been referenced in 1 publication.

    • Lau YF  et al. The magnitude of local immunity in the lungs of mice induced by live attenuated influenza vaccines is determined by local viral replication and induction of cytokines. J Virol 85:76-85 (2011). PubMed: 20962087

    Customer reviews and Q&As

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    Question

    Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

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    Answer

    Thank you for your enquiry.


    Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

    Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



    I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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