Rat monoclonal [LO-MM-9] Anti-Mouse IgM heavy chain (Biotin) (ab11659)

Overview

  • Product name
    Rat monoclonal [LO-MM-9] Anti-Mouse IgM heavy chain (Biotin)
    See all IgM secondary antibodies
  • Host species
    Rat
  • Target species
    Mouse
  • Specificity
    ab11659 recognises the mu chain of mouse IgM.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Polyclonal and monoclonal mouse IgM.

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Purified IgM
  • Purification notes
    Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B.
  • Clonality
    Monoclonal
  • Clone number
    LO-MM-9
  • Myeloma
    IR983F
  • Isotype
    IgG2a
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 µg/ml.

References

ab11659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cross adsorbed or tested against human IgM, and we cannot guarantee their suitable for your assay. Two other biotin conjugated anti-mouse IgG antibodies but only tested in ELISA: ab11659 Mouse IgM secondary antibody [LO-MM-9] - Heavy Chain specific. Unable to guarantee non-reactivity with human Ig. ab49760 will not recognise other mouse Ig's as there was no reaction against IgA, IgG, and IgM. Human IgG or IgM cross-reactivity has not been tested.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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