Rat monoclonal Anti-Mouse IgG2a heavy chain (HRP) [LO-MG2a-9] (ab11571)

Overview

  • Product name

    Rat monoclonal Anti-Mouse IgG2a heavy chain (HRP) [LO-MG2a-9]
    See all IgG2a secondary antibodies
  • Host species

    Rat
  • Target species

    Mouse
  • Specificity

    ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses.
  • Tested applications

    Suitable for: ELISAmore details
  • Immunogen

    Purified Mouse IgG2a heavy chain.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    LO-MG2a-9
  • Myeloma

    IR983F
  • Isotype

    IgG1
  • General notes

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

References

This product has been referenced in:

  • Ajina R  et al. SpCas9-expression by tumor cells can cause T cell-dependent tumor rejection in immunocompetent mice. Oncoimmunology 8:e1577127 (2019). Read more (PubMed: 31069138) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/index.html?datasheet=99603 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97237 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/index.html?datasheet=997240 Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/index.html?datasheet=11571 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97245 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97242 Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

Read More

Question
Answer

Thank you for your enquiry. Further to correspondence with the lab I can tell you that unfortunately this antibody has not been tested to determine its reactivity with other species' IgG2a. Following on we could therefore not guarantee it would not react with other species' IgG2a given a mixed sample. I am sorry that I cannot be of more assistance on this occasion.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up