Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)
![ELISA - Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)](https://www.abcam.com/ps/products/99/ab99589/Images/ab99589-272408-rat-monoclonal-sb73a-anti-mouse-igm-mu-chain-alkaline-phosphatase-elisa.jpg "ELISA - Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)")
Key features and details
- Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rat
- Isotype: IgG1
- Suitable for: Flow Cyt, ICC/IF, ELISA
Overview
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Product name
Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase)
See all IgM secondary antibodies -
Host species
Rat -
Target species
Mouse -
Specificity
ab99589 reacts with the µ chain of BALB/c mouse IgM as demonstrated by ELISA -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISAmore details -
Immunogen
Other Immunogen Type. Mouse IgM hybridoma
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SB73a -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent dilution.
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|
| ICC/IF |
Use at an assay dependent dilution.
|
|
| ELISA |
1/1000 - 1/2000.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent dilution. |
|
ICC/IF
Use at an assay dependent dilution. |
|
ELISA
1/1000 - 1/2000. |
Images
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ELISA - Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)ELISA standard curve. The ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with ab99589, serially diluted from a 1/1 dilution to a 1/8 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab99589 has not yet been referenced specifically in any publications.
