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Immunology Immunoglobulins Heavy Chain IgM
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Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)

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ELISA - Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)

    Key features and details

    • Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase)
    • Conjugation: Alkaline Phosphatase
    • Host species: Rat
    • Isotype: IgG1
    • Suitable for: Flow Cyt, ICC/IF, ELISA

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    Overview

    • Product name

      Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase)
      See all IgM secondary antibodies
    • Host species

      Rat
    • Target species

      Mouse
    • Specificity

      ab99589 reacts with the µ chain of BALB/c mouse IgM as demonstrated by ELISA
    • Tested applications

      Suitable for: Flow Cyt, ICC/IF, ELISAmore details
    • Immunogen

      Other Immunogen Type. Mouse IgM hybridoma

    • Conjugation

      Alkaline Phosphatase

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 8.00
      Preservative: 0.1% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      SB73a
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgM
      • Secondary antibodies
      • anti-Mouse
      • IgM
      • Enzyme
      • Alkaline Phos
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage

    Associated products

    • Alternative Versions

      • Rat F(ab')2 monoclonal [SB73a] Anti-Mouse IgM mu chain (ab99600)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab99589 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    Use at an assay dependent dilution.
    ELISA
    1/1000 - 1/2000.
    Notes
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    Use at an assay dependent dilution.
    ELISA
    1/1000 - 1/2000.

    Images

    • ELISA - Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)
      ELISA - Rat monoclonal [SB73a] Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab99589)
      ELISA standard curve. The ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with ab99589, serially diluted from a 1/1 dilution to a 1/8 dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab99589? Please let us know so that we can cite the reference in this datasheet.

    ab99589 has not yet been referenced specifically in any publications.

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