Overview

  • Product name

    Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed
    See all IgG1 secondary antibodies
  • Host species

    Rat
  • Target species

    Mouse
  • Specificity

    ab99604 reacts with Mouse IgG1 as demonstrated by ELISA. Minimal reaction to Human, Goat and Rabbit IgG. Each batch of Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.

  • Tested applications

    Suitable for: ELISA, Flow Cytmore details
  • Minimal
    cross-reactivity


    Goat, Human, Rabbit more details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SB77e
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/20000.
Flow Cyt Use at an assay dependent dilution.

Images

  • Top: (Negative control) ab99604 staining for Mouse IgG1 in Human blood at 1µg/106 cells.
    Bottom: ab99604 staining in Human blood incubated with mouse anti-Human CD3 (IgG1 isotype) and Streptavidin-FITC.

References

This product has been referenced in:

  • Park MH  et al. Development of a high-yield technique to isolate spermatogonial stem cells from porcine testes. J Assist Reprod Genet 31:983-91 (2014). Read more (PubMed: 24938360) »
See 1 Publication for this product

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