For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
We have recently purchased several antibodies from abcam. I'm the end user and was wondering if you possibly could supply a little more information about above products.
I have set up an assay to detect biotinylated DNA using your anti-biotin Ms antibody: ab79111. I detect a splendid shift on acrylamide gel when working with an oligo duplex but when working with larger DNA on agarose gel I don't see a nice band-to-band, instead it does look like a smear. Is this expected or should I assume the antibody is slowly coming off the biotinylated DNA during electrophoresis?
Do you perhaps know what is the binding constant between biotin and this antibody? How does it compare to biotin-streptavidin, I'm sure it's weaker but not sure how much. Do you know what is the overall charge of this protein and how many lysines does it contain?
My second question deals with your product: ab99602. Is concentration value available? I also wanted to check what is the molecule charge. What kind of binding should I expect between ab 79111 and ab99602, even a ballpark would be useful? Mostly do you expect it to be stronger than between epitope and primary antibody or weaker?
I'm nor very experienced working with antibodies and would greatly appreciate your feedback.
Asked on May 08 2012
Thank you for contacting Abcam.
I am not familiar with the assay that you are using and so I am not sure if I can answer your first question, but I will speak to some of my colleagues and see if there is anything they can suggest. I will let you know if I find out anything.
As for the second question, we do not have the concentration for this product available and I would expect that the binding between the antibodies would be as strong as that between the epitope and primary antibody.
Sorry I could not provide more information, but I will ask my colleagues and get back to you if I find anything else out.
Answered on May 09 2012