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Immunology Immunoglobulins Heavy Chain IgG
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Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (FITC) preadsorbed (ab133859)

  • Datasheet
  • SDS
Submit a review Q&A (2)

Product price, shipping and contact information

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Key features and details

  • Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rat
  • Isotype: IgG2b
  • Suitable for: ICC/IF, Flow Cyt, ELISA

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Overview

  • Product name

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (FITC) preadsorbed
    See all IgG1 secondary antibodies
  • Description

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (FITC) preadsorbed
  • Host species

    Rat
  • Target species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Goat, Human, Rabbit
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    SB77e
  • Isotype

    IgG2b
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Associated products

  • Alternative Versions

    • Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L preadsorbed (ab99601)
    • Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed (ab99602)
    • Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (HRP) preadsorbed (ab99603)
    • Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)
    • Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (ab99605)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use 1µg for 106 cells.
Flow Cyt
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
ICC/IF
Use 1µg for 106 cells.
Flow Cyt
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab133859? Please let us know so that we can cite the reference in this datasheet.

ab133859 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

vielen Dank für Ihre Hilfe und den Antikörper!

Könnten Sie mir noch einen Preis dazu nennen?



Vielen Dank für Ihre Mühen!

Read More

Abcam community

Verified customer

Asked on Oct 24 2012

Answer



Sie bekommen 250 ug für 180€ bei 21€ Versandtkosten. Die Preise können Sie ermitteln, indem Sie die Länderauswahl auf "Germany" stellen. Der Preis wird dann automatisch aktualisiert.

Read More

Abcam Scientific Support

Answered on Oct 24 2012

Question

vor einiger Zeit konnten Sie mir mit meinen Antikörpern (Collagen) helfen. Danke nochmal!



Jetzt suche ich einen Zweitantikörper für Immunhistochemie / Doppelfärbung.

Ich verwende zwei 1.Antikörper, wobei einer davon als 1.Antikörper

ein Plakophilin2 (2a+2b) Multi-epitope Cocktail Antikörper ist.

Category: Mouse monoclonal multi-epitope Cocktail

Ig Class: All IgG1



Der andere 1. Antikörper ist ein Goat IgG-Ak. Dafür habe ich einen passenden und schon

mehrfach getesteten 2.Ak (Anti-Goat IgG-Cy3).



Für den Plakophilin2-Ak suche ich einen 2.Ak der kein mouse oder rabbit ist. Gern einen

Donkey der aber FITC gelabelt sein muss!

Wenn es so einen Ak bei Abcam nicht gibt, würde ich auch einen Direkt gelabelten Plakophilin2-Ak

verwenden. Er müsste nur kompatible sein.





Folgende Doppelfärbung habe ich schon getestet:

Klappt

Anderer 1.Ak = Goat IgG

2.Ak = Goat IgG Cy3 rabbit



Klappt nicht:

1.Ak = PKP2 mouse IgG

2.Ak= Anti mouse FITC rabbit



Ich weiß, dass bei beiden 2.Ak ein rabbit Ak verwendet wurde und es “nur” ein Versuch war.

Leider hat dieser Versuch nicht geklappt.



Würde mich über eine Rückmeldung freuen!

Vielen Dank für Ihre Mühe!

Mit freundlichen Grüßen

Read More

Abcam community

Verified customer

Asked on Oct 23 2012

Answer

Vielen Dank für Ihren Anruf.

Wie am Telefon besprochen möchte ich Ihnen unseren ab133859 Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (FITC), pre-adsorbedempfehlen:

https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-FITC-preadsorbed-ab133859.html (or use the following: https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-FITC-preadsorbed-ab133859.html).

Ich finde es allerdings etwas bedenklich, dass Ihr ursprünglicher Anti-mous Ak nicht funktioniert, da dies in meinen Augen nicht am Versuchsaufbau liegen kann. Haben Sie schon überprüft, ob dieser AK in anderen Experimenten funktioniert hat?


Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Oct 23 2012

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