Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (HRP) preadsorbed (ab99603)

Overview

  • Product name

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (HRP) preadsorbed
    See all IgG1 secondary antibodies
  • Description

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (HRP) preadsorbed
  • Host species

    Rat
  • Target species

    Mouse
  • Specificity

    ab99603 has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins.
  • Tested applications

    Suitable for: ELISAmore details
  • Minimal
    cross-reactivity


    Goat, Human, Rabbit more details
  • Conjugation

    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab99603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000 - 1/8000.

References

This product has been referenced in:

  • Villadiego J  et al. Immunization with a-synuclein/Grp94 reshapes peripheral immunity and suppresses microgliosis in a chronic Parkinsonism model. Glia 66:191-205 (2018). Read more (PubMed: 29024008) »
  • Kanda K  et al. Detection of titin fragments in urine in response to exercise-induced muscle damage. PLoS One 12:e0181623 (2017). Read more (PubMed: 28727760) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/index.html?datasheet=99603 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97237 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/index.html?datasheet=997240 Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/index.html?datasheet=11571 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97245 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97242 Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

Read More

Answer

Thank you for your enquiry and interest in our products. This product is not affinity purified so we are unable to provide the exact IgG values since it is not quantified. However, as our datasheet indicates we could recommend this product in ELISA at this dilution range:1/4000 - 1/8000. Please do not hesitate to contact me again if you need any further assistance.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up