Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) (ab172324)

Overview

  • Product name

    Rat monoclonal [SB84a] Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488)
    See all IgG2a secondary antibodies
  • Host species

    Rat
  • Target species

    Mouse
  • Specificity

    This antibody is specific to Mouse IgG2a heavy chain
  • Tested applications

    Suitable for: IHC-Fr, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Clone number

    (SB84a)
  • Isotype

    IgG1
  • Light chain type

    kappa
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/1000 - 1/2000.

ab171464 - Mouse monoclonal IgG2a (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

 

Images

  • Overlay histogram showing subclass specificity of ab172324. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22°C. The secondary antibody (ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a, heavy chain - Alexa Fluor® 488) was used at 1/1000 dilution for 30 min at 22ºC. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

References

ab172324 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Protocol:
1. Tissue was fixed in cold acetone for 10 minutes
2. Tissue was blocked with 1% BSA, 0.1% in PBS (Blocking Buffer) for 30 minutes
3. Tissue was stained with first antibody pure mouse-anti-human HLA-DR overnight in Blocking Buffer
4. After washing, Tissue was stained with ab172324 - Rat monoclonal (SB84a) Anti-Mouse IgG2a heavy chain (Alexa Fluor® 488) for 2 hours in Blocking Buffer.

Abcam user community

Verified customer

Submitted Jun 18 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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