Rat Myoglobin ELISA Kit (ab157739)

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Standard Curve

    Key features and details

    • Sensitivity: 3.365 ng/ml
    • Range: 6.25 ng/ml - 200 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

    Overview

    • Product name

      Rat Myoglobin ELISA Kit
      See all Myoglobin kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%

    • Sample type

      Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      3.365 ng/ml

    • Range

      6.25 ng/ml - 200 ng/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Rat

    • Product overview

      Abcam’s Myoglobin Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin levels in rat serum and plasma.

      In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    Images

    • Standard Curve
      Standard Curve
      Representative standard curve using ab157739 Myoglobin Rat ELISA Kit.

    References (1)

    Publishing research using ab157739? Please let us know so that we can cite the reference in this datasheet.

    ab157739 has been referenced in 1 publication.

    • Zuo Q  et al. Response of macrophages in rat skeletal muscle after eccentric exercise. Chin J Traumatol 21:88-95 (2018). PubMed: 29550244

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