Rat Myoglobin ELISA Kit (ab157739)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Rat Myoglobin ELISA Kit
See all Myoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.365 ng/ml -
Range
6.25 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s Myoglobin Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin levels in rat serum and plasma.
In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-rat Myoglobin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat Myoglobin Calibrator (lyophilized) 1 vial Rat Myoglobin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Function
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
Sequence similarities
Belongs to the globin family. - Information by UniProt
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Alternative names
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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Database links
- Entrez Gene: 59108 Rat
- SwissProt: Q9QZ76 Rat
- Unigene: 40511 Rat
Images
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Standard CurveRepresentative standard curve using ab157739 Myoglobin Rat ELISA Kit.
Protocols
References
This product has been referenced in:
- Zuo Q et al. Response of macrophages in rat skeletal muscle after eccentric exercise. Chin J Traumatol 21:88-95 (2018). Read more (PubMed: 29550244) »
