Overview

  • Product name
    Rat Myoglobin ELISA Kit
    See all Myoglobin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    3.365 ng/ml
  • Range
    6.25 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Myoglobin Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin levels in rat serum and plasma.

    In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab157739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard Curve
    Standard Curve
    Representative standard curve using ab157739 Myoglobin Rat ELISA Kit.

Protocols

References

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