Rat Myoglobin ELISA Kit (ab157739)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameRat Myoglobin ELISA Kit
See all Myoglobin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeSerum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity3.365 ng/ml
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Range6.25 ng/ml - 200 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Rat
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Product overview
Abcam’s Myoglobin Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin levels in rat serum and plasma.
In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsPlease refer to protocols.
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Components 1 x 96 tests 100X HRP-conjugated anti-rat Myoglobin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat Myoglobin Calibrator (lyophilized) 1 vial Rat Myoglobin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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FunctionServes as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
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Sequence similaritiesBelongs to the globin family.
- Information by UniProt
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Alternative names
- MB
- MGC13548
- MYG_HUMAN
see all -
Database links
- Entrez Gene: 59108 Rat
- SwissProt: Q9QZ76 Rat
- Unigene: 40511 Rat
Applications
Our Abpromise guarantee covers the use of ab157739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Standard CurveRepresentative standard curve using ab157739 Myoglobin Rat ELISA Kit.
Protocols
References
This product has been referenced in:
- Zuo Q et al. Response of macrophages in rat skeletal muscle after eccentric exercise. Chin J Traumatol 21:88-95 (2018). Read more (PubMed: 29550244) »
