Overview

  • Product name

    Rat Neurotrophin-3 ELISA Kit
    See all Neurotrophin 3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 94pg/ml 4.42 4.7%
    Sample 2 16 382pg/ml 20.63 5.4%
    Sample 3 16 624pg/ml 37.44 6%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 123pg/ml 6.4 5.2%
    Sample 2 24 417pg/ml 24.2 5.8%
    Sample 3 24 716pg/ml 50.84 7.1%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Quantitative
  • Sensitivity

    < 2 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Rat Neurotrophin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213905) is designed for the quantitative measurement of Rat Neurotrophin-3 in cell culture supernatants and serum. 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neurotrophin-3 amount of sample captured in plate.

  • Notes

    Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-rat Neurotrophin-3 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Rat Neurotrophin-3 antibody 1 x 130µl
    Lyophilized recombinant Rat Neurotrophin-3 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Seems to promote the survival of visceral and proprioceptive sensory neurons.
  • Tissue specificity

    Brain and peripheral tissues.
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • HDNF
    • MGC129711
    • Nerve growth factor 2
    • Neurotrophic factor
    • Neurotrophin 3 precursor
    • Neurotrophin-3
    • Neurotrophin3
    • NGF 2
    • NGF-2
    • NGF2
    • NT 3
    • NT-3
    • NT3
    • NTF 3
    • NTF3
    • NTF3_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat Neurotrophin-3 ELISA Kit (ab213905) Standard Curve.

Protocols

References

ab213905 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213905.
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