Overview

  • Product name

    Rat Osteopontin ELISA kit
    See all Osteopontin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.02 ng/ml
  • Range

    0.06 ng/ml - 4 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 0.06ng/ml - 4ng/ml

  • Assay time

    3h 20m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Osteopontin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Osteopontin protein in rat plasma, serum and urine.

  • Notes

    In this assay the Osteopontin present in samples reacts with the anti-Osteopontin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Osteopontin, is added and complexes are formed. Following a wash step, horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Osteopontin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Osteopontin in the test sample. The quantity of Osteopontin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab205076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205076 Rat Osteopontin ELISA Kit.

Protocols

References

ab205076 has not yet been referenced specifically in any publications.

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