Rat PAI1 ELISA Kit (SERPINE1) (ab198509)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Rat PAI1 ELISA Kit (SERPINE1)
See all PAI1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Known Conc 20 0.957ng/ml 0.088 9.24% Known Conc 20 2.25ng/ml 0.134 5.97% Known Conc 20 15.6ng/ml 1.01 6.48% Inter-assay Sample n Mean SD CV% Known Conc 10 0.926ng/ml 0.113 12.2% Known Conc 10 1.4ng/ml 0.096 6.86% Known Conc 10 9.17ng/ml 0.858 9.36% -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
0.079 ng/ml -
Range
0.1 ng/ml - 50 ng/ml -
Recovery
104 %
Sample specific recovery Sample type Average % Range Spike 104 97% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat
Does not react with: Sheep -
Product overview
Abcam’s PAI1 (SERPINE1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active PAI1 (SERPINE1) in rat plasma and serum.
Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate and will not be detected. Unbound PAI1 samples are washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI1 present in the samples, is then bound by the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-rabbit horseradish peroxidase secondary antibody 1 vial Anti-rat PAI-1 primary antibody 1 vial Rat PAI-1 activity standard 1 vial TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests -
Research areas
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Function
Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. -
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Endothelial plasminogen activator inhibitor
- PAI
- PAI-1
see all -
Database links
- Entrez Gene: 24617 Rat
- SwissProt: Q6AXS5 Rat
- Unigene: 29367 Rat
Applications
Our Abpromise guarantee covers the use of ab198509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Standard CurveFull range standard curve using ab198509 PAI1 (SERPINE1) Rat ELISA Kit.
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Standard CurveLinear range standard curve using ab198509 PAI1 (SERPINE1) Rat ELISA Kit.
Protocols
References
This product has been referenced in:
- Grasa-López A et al. Undaria pinnatifida and Fucoxanthin Ameliorate Lipogenesis and Markers of Both Inflammation and Cardiovascular Dysfunction in an Animal Model of Diet-Induced Obesity. Mar Drugs 14:N/A (2016). Rat . Read more (PubMed: 27527189) »
- Jiang H et al. Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies. Sci Rep 6:30464 (2016). Rat . Read more (PubMed: 27456456) »
