Rat PAI1 ELISA Kit (SERPINE1) (ab198509)

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Standard Curve
  • Standard Curve

Key features and details

  • Sensitivity: 0.079 ng/ml
  • Range: 0.1 ng/ml - 50 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Quantitative
  • Reacts with: Rat

Overview

  • Product name

    Rat PAI1 ELISA Kit (SERPINE1)
    See all PAI1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Known Conc 20 0.957ng/ml 0.088 9.24%
    Known Conc 20 2.25ng/ml 0.134 5.97%
    Known Conc 20 15.6ng/ml 1.01 6.48%
    Inter-assay
    Sample n Mean SD CV%
    Known Conc 10 0.926ng/ml 0.113 12.2%
    Known Conc 10 1.4ng/ml 0.096 6.86%
    Known Conc 10 9.17ng/ml 0.858 9.36%

  • Sample type

    Serum, Plasma

  • Assay type

    Quantitative

  • Sensitivity

    0.079 ng/ml

  • Range

    0.1 ng/ml - 50 ng/ml

  • Recovery

    104 %

    Sample specific recovery
    Sample type Average % Range
    Spike 104 97% - 108%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Rat
    Does not react with: Sheep

  • Product overview

    Abcam’s PAI1 (SERPINE1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active PAI1 (SERPINE1) in rat plasma and serum.

     

    Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate and will not be detected. Unbound PAI1 samples are washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI1 present in the samples, is then bound by the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-rabbit horseradish peroxidase secondary antibody 1 vial
    Anti-rat PAI-1 primary antibody 1 vial
    Rat PAI-1 activity standard 1 vial
    TMB Substrate Solution 1 x 10ml
    uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests

  • Research areas

  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

  • Sequence similarities

    Belongs to the serpin family.

  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • Nexin plasminogen activator inhibitor type 1
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serine proteinase inhibitor clade E member 1
    • serpin
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all

  • Database links

    Associated products

      Images

      • Standard Curve
        Standard Curve

        Full range standard curve using ab198509 PAI1 (SERPINE1) Rat ELISA Kit.

      • Standard Curve
        Standard Curve

        Linear range standard curve using ab198509 PAI1 (SERPINE1) Rat ELISA Kit.

      References (2)

      Publishing research using ab198509? Please let us know so that we can cite the reference in this datasheet.

      ab198509 has been referenced in 2 publications.

      • Grasa-López A  et al. Undaria pinnatifida and Fucoxanthin Ameliorate Lipogenesis and Markers of Both Inflammation and Cardiovascular Dysfunction in an Animal Model of Diet-Induced Obesity. Mar Drugs 14:N/A (2016). Rat . PubMed: 27527189
      • Jiang H  et al. Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies. Sci Rep 6:30464 (2016). Rat . PubMed: 27456456

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