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|Sample type||Average %||Range|
|Serum||100||91% - 108%|
|Plasma||101.6||84% - 114%|
|Cell culture media||130.4||122% - 138%|
Abcam’s PDGF-AA Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat PDGF-AA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for rat PDGF-AA coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat PDGF-AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|280X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-rat PDGF-AA||2 vials|
|PDGF-AA Microplate (12 x 8 wells)||1 unit|
|Recombinant Rat PDGF-AA Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"