Key features and details
- Sensitivity: 90 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat PDGF-AA ELISA Kit
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12%
Sample typeSerum, Plasma, Cell culture media
Assay typeSandwich (quantitative)
Sensitivity< 90 pg/ml
Range93.75 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Serum 100 91% - 108% Plasma 101.6 84% - 114% Cell culture media 130.4 122% - 138%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s PDGF-AA Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat PDGF-AA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for rat PDGF-AA coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat PDGF-AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 280X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-rat PDGF-AA 2 vials PDGF-AA Microplate (12 x 8 wells) 1 unit Recombinant Rat PDGF-AA Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceFunction: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Subunit structure: Homodimer (AA); antiparallel disulfide-linked dimer. Heterodimer with PDGFB (AB); antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4.
- PDGF A
- PDGF A chain
- PDGF subunit A
ab155464 has been referenced in 1 publication.
- Li X et al. Comparison of subacute and chronic scar tissues after complete spinal cord transection. Exp Neurol 306:132-137 (2018). PubMed: 29753649