Overview

  • Product name

    Rat PDGF-AB ELISA Kit
    See all PDGF B kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 246pg/ml 14.27 5.8%
    Sample 2 16 632pg/ml 34.76 5.5%
    Sample 3 16 1068pg/ml 50.2 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 275pg/ml 20.35 7.4%
    Sample 2 24 723pg/ml 45.55 6.3%
    Sample 3 24 1249pg/ml 73.7 5.9%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 3 pg/ml
  • Range

    31.2 pg/ml - 2000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Rat PDGF-AB Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213906) is designed for the quantitative measurement of Rat PDGF-AB in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-AB has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat PDGF-AB amount of sample captured in plate.

  • Notes

    The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.  The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.  PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF Bchain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat PDGF-ABELISA Kit (ab213906) Standard Curve

Protocols

References

ab213906 has not yet been referenced specifically in any publications.

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