Key features and details
- Sensitivity: 8 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat PDGF BB ELISA Kit
See all PDGF B kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range8.19 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Serum 120.7 115% - 127% Plasma 100 93% - 108% Cell culture media 111.5 101% - 121%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Rat PDGF BB ELISA Kit is designed for the quantitative determination of PDGF BB in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for rat PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects rat and human PDGF BB.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Anti-Rat Rat PDGF BB coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rat Rat PDGF BB Detection Antibody 2 vials Rat Rat PDGF BB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
- Information by UniProt
- c sis
ab267585 has not yet been referenced specifically in any publications.