Overview

  • Product name

    Rat Prolactin R ELISA Kit (PRLR)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.4 ng/ml
  • Range

    0.512 ng/ml - 50 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 125.4 117% - 141%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s Prolactin R Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Prolactin R in cell culture supernatants.


    This assay employs an antibody specific for rat Prolactin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Prolactin R antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat Prolactin R in normal serum with ab113351, only in serum spiked with Rat Prolactin R.

  • Notes

    Note: very low signal were obtained in normal plasma.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Biotinylated anti-rat Prolactin R 2 vials
    Prolactin R Microplate (12 x 8 wells) 1 unit
    Recombinant rat Prolactin R Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • Tissue specificity

    Expressed in breast, placenta, kidney, liver and pancreas.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Secreted and Membrane.
  • Information by UniProt
  • Alternative names

    • AI987712
    • CLONE SPM213
    • CPRLP
    • Delta 4-delta 7/11 truncated prolactin receptor
    • Delta 4-SF1b truncated prolactin receptor
    • hPRL receptor
    • hPRLrI
    • Lactogen receptor
    • MGC105486
    • OPR
    • OTTHUMP00000115998
    • Pr-1
    • PRL R
    • PRL-R
    • PRLR
    • Prlr-rs1
    • PRLR_HUMAN
    • Prolactin receptor
    • Prolactin receptor a
    • Prolactin receptor delta 7/11
    • RATPRLR
    • Secreted prolactin binding protein
    • wu:fj65c07
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab113351

Protocols

References

This product has been referenced in:

  • Adedara IA  et al. Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Arch Environ Contam Toxicol N/A:N/A (2014). Read more (PubMed: 25179371) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your interest in our Prolactin R Rat ELISA, ab113351. The laboratory that developed the ELISA has not tested it on milk or pituitary samples, but does not expect that there will be any problems. We recommend following the kit protocol suggestions for preparation of other samples types, using the same kit buffers. If you have any questions, please let us know.

Read More

Question
Answer

The laboratory has confirmed that this kit will not detect mouse Prolactin R. The rabbit polyclonal capture antibody will, but not the mouse monoclonal detection antibody.

Read More

Answer

Gracias por contactarnos.


El anticuerpo usado como captura cubriendo la placa procede de cabra, y es policlonal. El otro anticuerpo, el de detección se ha producido en ratón, y es monoclonal.

Espero haberte ayudado. Para más consultas, por favor, no dudes en volvernos a contactar.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up