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Immunology Innate Immunity Chemokines Beta Chemokines (CC)
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Rat Rantes ELISA Kit (ab213907)

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Rat Rantes ELISA Kit (ab213907) Standard Curve

    Key features and details

    • Sensitivity: 1 pg/ml
    • Range: 15.6 pg/ml - 1000 pg/ml
    • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

    You may also be interested in

    Protein
    Product image
    Recombinant rat RANTES protein (ab78608)

    View more associated products

    Overview

    • Product name

      Rat Rantes ELISA Kit
      See all RANTES kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Sample 1 16 135pg/ml 6.21 4.6%
      Sample 2 16 327pg/ml 17 5.2%
      Sample 3 16 536pg/ml 29.48 5.5%
      Inter-assay
      Sample n Mean SD CV%
      Sample 1 24 118pg/ml 9.68 8.2%
      Sample 2 24 293pg/ml 19.63 6.7%
      Sample 3 24 625pg/ml 43.75 7%
    • Sample type

      Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 1 pg/ml
    • Range

      15.6 pg/ml - 1000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      The Rat Rantes Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213907) is designed for the quantitative measurement of Rat Rantes in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). 


      The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rantes has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Rantes amount of sample captured in plate.

    • Notes

      Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete Rantes. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent. 

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-rat Rantes coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Rat Rantes antibody 1 x 130µl
      Lyophilized recombinant Rat Rantes standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Chemokines
      • Beta Chemokines (CC)
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Chemokines
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV-related
    • Function

      Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
    • Tissue specificity

      T-cell and macrophage specific.
    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications

      N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
      The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P13501 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Beta chemokine RANTES
      • Beta chemokine RANTES precursor
      • C C motif chemokine 5
      • CCL 5
      • CCL5
      • CCL5_HUMAN
      • Chemokine (C C motif) ligand 5
      • Chemokine CC Motif Ligand 5
      • D17S136E
      • EoCP
      • Eosinophil chemotactic cytokine
      • MGC17164
      • RANTES(4-68)
      • Regulated upon activation normally T expressed and presumably secreted
      • SCYA 5
      • SCYA5
      • SIS delta
      • SIS-delta
      • SISd
      • Small inducible cytokine A5
      • Small inducible cytokine A5 (RANTES)
      • Small inducible cytokine subfamily A (Cys Cys) member 5
      • Small-inducible cytokine A5
      • T cell specific protein p288
      • T cell specific protein RANTES
      • T cell specific RANTES protein
      • T cell-specific protein P228
      • T-cell-specific protein RANTES
      • TCP 228
      • TCP228
      see all
    • Database links

      • Entrez Gene: 81780 Rat
      • SwissProt: P50231 Rat
      • Unigene: 8019 Rat

      Associated products

        Images

        • Rat Rantes ELISA Kit (ab213907) Standard Curve
          Rat Rantes ELISA Kit (ab213907) Standard Curve
          Rat Rantes ELISA Kit (ab213907) Standard Curve

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab213907? Please let us know so that we can cite the reference in this datasheet.

        ab213907 has not yet been referenced specifically in any publications.

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