Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat sE-Selectin ELISA Kit (sCD62E)
See all CD62E kits
Intra-assay Sample n Mean SD CV% 1 16 345pg/ml 17.68 = 4.4% 2 16 1737pg/ml 132.01 = 7.6% 3 16 4428pg/ml 292.24 = 6.6% Inter-assay Sample n Mean SD CV% 1 24 14.8pg/ml 14.8 = 4.7% 2 24 149.13pg/ml 149.13 = 9.2% 3 24 309.85pg/ml 309.85 = 7.6%
Sample typeCell culture supernatant, Serum, Hep Plasma, Cit plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range156 pg/ml - 10000 pg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s rat soluble sE-Selectin (sCD62E) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat soluble sE-Selectin in cell culture supernatants, serum and plasma (heparin, citrate).
An sE-Selectin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for sE-Selectin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat sE-Selectin amount of sample captured in plate.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat sE-Selectin Antibody Microplate (12 x 8 wells) 1 x 96 tests Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-rat sE-Selectin antibody 1 x 130µl Lyophilized recombinant rat sE-Selectin standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
- Information by UniProt
- CD62 antigen-like family member E
- CD62E antigen
ab171334 has been referenced in 1 publication.
- Mahmoud-Awny M et al. Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-?, NF-?B and Nrf2/HO-1 Signaling Pathways. PLoS One 10:e0132497 (2015). PubMed: 26196679