Overview

  • Product name
    Rat Serum Amyloid P ELISA Kit (APCS)
    See all Serum Amyloid P kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    4.01 ng/ml
  • Range
    2.5 ng/ml - 160 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Serum Amyloid P (APCS) Rat ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Serum Amyloid P levels in rat serum and plasma samples.

     

    In this assay the Serum Amyloid P present in samples reacts with the anti-Serum Amyloid P antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Serum Amyloid P antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Serum Amyloid P. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Serum Amyloid P in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Serum Amyloid P in the test sample. The quantity of Serum Amyloid P in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat Plasminogen antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Rat Serum Amyloid P Calibrator (lyophilized) 1 vial
    Rat Serum Amyloid P ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity
    Found in serum and urine.
  • Involvement in disease
    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • 9.5S alpha-1-glycoprotein
    • APCS
    • SAMP_HUMAN
    • SAP
    • Serum amyloid P-component(1-203)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157741 Serum Amyloid P Rat ELISA Kit.

Protocols

References

ab157741 has not yet been referenced specifically in any publications.

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