Description

Associated products

Specifications

Our Abpromise guarantee covers the use of ab38217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form

    Liquid
  • Additional notes

    Peptide MW is 1452 daltons.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: Whole serum

General Info

  • Alternative names

    • C-terminal-flanking peptide
    • Hs.2563
    • neurokinin 1
    • neurokinin 2
    • neurokinin A
    • neurokinin alpha
    • Neuromedin L
    • neuropeptide gamma
    • neuropeptide K
    • NK2
    • NKA
    • NKNA
    • NPK
    • PPT
    • preprotachykinin
    • protachykinin
    • protachykinin-1
    • Substance K
    • SubstanceP
    • TAC1
    • TAC2
    • TAC2, formerly
    • Tachykinin 1
    • tachykinin 2
    • tachykinin 2, formerly
    • Tachykinin precursor 1
    • tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)
    • Tachykinin1
    • TKN1_HUMAN
    see all
  • Function

    Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
  • Sequence similarities

    Belongs to the tachykinin family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab38217 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.

As I had mentioned, we have not validated ab14184 for use on frozen sections. Not all antibodies will work for every application, and for that reason we only cover tested applications and species under our Abpromise guarantee.

While antibodies that have been validated for use on paraffin-embedded sections may work with frozen sections, they are not always compatible. The main difference between these assays is the use of antigen retrieval. For any formaldehyde-based fixation performed for longer than 20 minutes, the proteins will be cross-linked. If the epitope recognized by the antibody is obscured by the methylene bridges formed by formaldehyde fixation, no specific binding will be observed. Antigen retrieval will partially digest the methylene bridges and expose more epitopes in the protein. While this is important for polyclonal antibodies, it can be especially crucial for monoclonal antibodies that recognize only one epitope. Our Substance P antibody ab14184 has not been tested on cross-linked samples without the use of an antigen retrieval step.

If you are ever interested in testing an antibody in an untested species or application, please feel free to inquire about our 100% Testing Discount prior to purchase:

https://www.abcam.com/collaborationdiscount

I would be happy to help you troubleshoot this further to improve your IHC-Fr results, or to offer you a refund or credit if the antibody does not work in one of our tested applications: ELISA, IHC-P, or ICC/IF. Please let me know if you have any further questions or concerns.

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Answer

Thank you for contacting us. I am sorry that ab14184 is giving non-specific staining in IHC-Fr. We have not validated this antibody for use on frozen sections, but it should be suitable for that purpose with some troubleshooting.

1) What species did your samples come from?

2) Could you please let me know the dilutions and incubation times used for the primary and secondary antibodies? Have you performed a no-primary antibody control to rule out the secondary antibody as the cause of the background staining? It may help to reduce the secondary antibody concentration to around 1:500 - 1:1000.

3) Were the sections fixed with 4% PFA for 20 minutes or just cryoprotected with the sucrose treatment? We have been able to obtain staining using a 4% PFA fixation with Proteinase K antigen retrieval or heat mediated antigen retrieval. It may help to add a 10 minuteProteinase K treatment to your protocol prior to blocking. Alternatively, you could perform the sucrose cryoprotection followed by methanol fixation.

4) To ensure that the excess antibody is washed off, I would recommend doing 3 x 5 minute washes in PBST.

5) Since you appear to be getting a lot of background staining, you could try blocking with 10% normal goat serum for a longer period of time. Usually30 minutes to an hour is sufficient, butincubating overnight with the blocking agent canhelp to further reduce the background.

I hope this helps, if not, please let me know and I will be happy to help you further.

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