Rat TGF beta 1 ELISA Kit (ab119558)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameRat TGF beta 1 ELISA Kit
See all TGF beta 1 kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Serum 4 6.8% Inter-assay Sample n Mean SD CV% Plasma 4 6.8% -
Sample typeCell culture supernatant, Serum, Plasma, Tissue Lysate
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Assay typeSandwich (quantitative)
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Sensitivity8 pg/ml
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Range31.3 pg/ml - 2000 pg/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 95% - 118% Serum 100 95% - 106% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Rat
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Product overview
Abcam’s TGF beta 1 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Rat TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA and heparin).
Rat TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Rat TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rat TGF beta 1 captured on the plate.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 20X Assay Buffer Concentrate 2 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Biotin-Conjugate anti-rat TGF beta 1 monoclonal antibody 1 x 120µl Microplate coated with monoclonal antibody to rat TGF beta 1 (12 x 8 wells) 1 unit Plate seal 6 units Rat TGF beta 1 Standard Lyophilized 2 vials Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
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Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
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Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
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Sequence similaritiesBelongs to the TGF-beta family.
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Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Alternative names
- Camurati-Engelmann disease
- CED
- DPD1
see all -
Database links
- Entrez Gene: 59086 Rat
- SwissProt: P17246 Rat
- Unigene: 40136 Rat
Applications
Our Abpromise guarantee covers the use of ab119558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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ELISA: TGF beta 1 Rat ELISA Kit (ab119558)Activated TGF-beta 1 measured in tissue lysates, homogenised in RIPA buffer, activated (20 uL of HCl per 20 uL of samples with 180 uL of buffer) and tested between 25-250 ug protein per mL. Data expressed as TGF beta per mg of extracted protein (duplicates +/- SD).
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ELISA: TGF beta 1 Rat ELISA Kit (ab119558)Activated TGF-beta 1 measured in rat biological fluids (dilution range 1/50-1/200; duplicates +/- SD). Human and mouse samples, diluted 1:12, gave results below the level of detection.
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Sandwich ELISA - TGF beta 1 Rat ELISA Kit (ab119558)This image is courtesy of an anonymous AbreviewSandwich ELISA on rat blood serum using Abcam's TGF beta 1 Rat ELISA Kit (ab119558).
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Typical Standard CurveRepresentative Standard Curve using ab119558.
Protocols
References
This product has been referenced in:
- Sansoè G et al. Role of Chymase in the Development of Liver Cirrhosis and Its Complications: Experimental and Human Data. PLoS One 11:e0162644 (2016). Read more (PubMed: 27637026) »
- Monaghan M et al. A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling. Mol Ther 22:786-96 (2014). Read more (PubMed: 24402185) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"