Rat TGF beta 1 ELISA Kit (ab119558)

Thank you for contacting us.

Here is the correct protocol section:

Prepare your serum and plasma samples before starting the test
procedure. Dilute serum and plasma samples with Assay Buffer (1x)
according to the following scheme:
- 20 μl sample + 920 μl Assay Buffer (1x)
- Add 30 μl 1N HCl to 940 μl prediluted sample, mix and incubate for
1 hour at room temperature.
- Neutralize by addition of 30 μl 1N NaOH.
Prepare your cell culture supernatant samples before starting the test
procedure. Dilute cell culture supernatant samples with Assay Buffer
(1x) according to the following scheme:
- 20 μl sample + 180 μl Assay Buffer (1x)
- Add 20 μl 1N HCl to 200 μl prediluted sample, mix and incubate for
1 hour at room temperature.
- Neutralize by addition of 20 μl 1N NaOH.

The table is correct.

I am very sorry for this mistake and the confusion caused.
Please let me know how the results turned out.

If the data are incorrect or do not make any sense, please let me know your order or PO number as we made a mistake on our end and would like to remedy that by replacing the kit free of charge.
I look forward to hear back from you.

We have not tested bronchoalveolar lavage fluid with ab119558, so we do not have a recommended dilution for this sample type. If you perform a literatire search to determine the expected levels of TGF beta 1 in this fluid compared to serum, plasma, or cell culture supernatant, then you can use the expected level as a guide to what dilution to use.

We always recommend testing multiple different dilutions of your sample to ensure that the readings fall within the range of the standard curve.

Thank you for contacting us.


Our TGF beta 1 Rat ELISA Kit will detect the inactive ( which get activated during the sample preparation (HCL and NaOH)) and active form.

It will not detect the LAP complex.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.
I can confirm that the volume to be added is 400 ul.
The "2 x" is an oversight on our end due to the fact that 2 vials are being sent.
I will look into having this corrected.
Thank you for pointing this out to us.
Please do not hesitate to contact us if you need any more advice or information.
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Thank you again for your patience.

I've just heard back from the lab, andstandard should be reconstituted in 400 ulto a concentration of400 pg/ml.

Please let me know if you have further questions or if there is anything else we can do. Have a great day.

Thank you for your call yesterday, and I apologize again for the missing protocol information.

I just wanted to touch base and let you know that I'm still waiting for this information from the lab. I will forward it on to you as soon as possible! Please let me know if there is anything else that we can do for you in the meantime, and we appreciate your patience.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. The estimated delivery date is xxx. I apologize about this delay.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.