• Product name
    Rat TGF beta 1 ELISA Kit
    See all TGF beta 1 kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Serum 4 6.8%
    Sample n Mean SD CV%
    Plasma 4 6.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue Lysate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    8 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105 95% - 118%
    Serum 100 95% - 106%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s TGF beta 1 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Rat TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA and heparin).

    Rat TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Rat TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rat TGF beta 1 captured on the plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 2 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Biotin-Conjugate anti-rat TGF beta 1 monoclonal antibody 1 x 120µl
    Microplate coated with monoclonal antibody to rat TGF beta 1 (12 x 8 wells) 1 unit
    Plate seal 6 units
    Rat TGF beta 1 Standard Lyophilized 2 vials
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Activated TGF-beta 1 measured in tissue lysates, homogenised in RIPA buffer, activated (20 uL of HCl  per 20 uL of samples with 180 uL of buffer) and tested between 25-250 ug protein per mL. Data expressed as TGF beta per mg of extracted protein (duplicates +/- SD).

  • Activated TGF-beta 1 measured in rat biological fluids (dilution range 1/50-1/200; duplicates +/- SD). Human and mouse samples, diluted 1:12, gave results below the level of detection.

  • Sandwich ELISA on rat blood serum using Abcam's TGF beta 1 Rat ELISA Kit (ab119558).

  • Representative Standard Curve using ab119558.



This product has been referenced in:
  • Sansoè G  et al. Role of Chymase in the Development of Liver Cirrhosis and Its Complications: Experimental and Human Data. PLoS One 11:e0162644 (2016). Read more (PubMed: 27637026) »
  • Monaghan M  et al. A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling. Mol Ther 22:786-96 (2014). Read more (PubMed: 24402185) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Here is the correct protocol section:

Prepare your serum and plasma samples before starting the test
procedure. Dilute serum and plasma samples with Assay Buffer (1x)
according to the followi...

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We have not tested bronchoalveolar lavage fluid with ab119558, so we do not have a recommended dilution for this sample type. If you perform a literatire search to determine the expected levels of TGF beta 1 in this fluid compared to serum, plasma, or ...

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TGF-beta ELISA assay kit in rat blood serum is very very excellent and easy step.
But, color development is very quickley disappears after stop solution.

Abcam user community

Verified customer

Submitted Mar 04 2014

Thank you for contacting us.

Our TGF beta 1 Rat ELISA Kit will detect the inactive ( which get activated during the sample preparation (HCL and NaOH)) and active form.

It will not detect the LAP complex.


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Thank you for contacting us.
I can confirm that the volume to be added is 400 ul.
The "2 x" is an oversight on our end due to the fact that 2 vials are being sent.
I will look into having this corrected.
Thank you for pointing this ...

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Thank you again for your patience.

I've just heard back from the lab, andstandard should be reconstituted in 400 ulto a concentration of400 pg/ml.

Please let me know if you have further questions or if there is anything else we can ...

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Thank you for your call yesterday, and I apologize again for the missing protocol information.

I just wanted to touch base and let you know that I'm still waiting for this information from the lab. I will forward it on to you as soon as possi...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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