Overview

  • Product name

    Rat TIMP-2 ELISA Kit
    See all TIMP2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.17ng/ml 0.051 4.4%
    2 16 2.9ng/ml 0.168 5.8%
    3 16 5.85ng/ml 0.368 6.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.21ng/ml 0.091 7.5%
    2 24 3.55ng/ml 0.256 7.2%
    3 24 6.38ng/ml 0.408 6.4%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Rat TIMP-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213923) is designed for the quantitative measurement of Rat TIMP-2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat TIMP-2 amount of sample captured in plate.

  • Notes

    TIMP-2 gene is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat TIMP-2 ELISA Kit (ab213923) Standard Curve.

Protocols

References

ab213923 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
The samples: Urine from rats, treated with an antitumor drug, which causes acute kidney damage. Samples of the rats treated with the drug are taken at different points in time to observe the evolution of acute kidney damage. Later they are compared with the samples of the control rats, taken in the same points in time as the treated ones.
Samples are stored at -80 0C when they are to be used at a time after collection, and at -20 0C can be used within the next 30 days. These samples were stored at -80 and later were removed and aliquoted for the experiment, keeping at -20 0C.
First, we did a dilution test, where we used samples of the different study points of treated rats and controls. Undiluted, 1: 2, 1: 5, 1:10. For the principal experiment, we have taken the following dilutions: controls and basal of the treated 1: 2, treated with the drug 1:10.
Results of the experiment.
Rats treated with the drug (with kidney damage) have higher levels of TIMP2 initially after treatment, which later decreases to the values observed in untreated rats and their basal value.

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Verified customer

Submitted Jul 17 2019

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