Key features and details
- Sensitivity: 0.043 ng/ml
- Range: 0.05 ng/ml - 50 ng/ml
- Sample type: Other biological fluids, Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Rat
Product nameRat Tissue Plasminogen Activator ELISA Kit (TPA)
See all PAI1 + tPA kits
Intra-assay Sample n Mean SD CV% Known Conc 20 0.8ng/ml 0.034 4.28% Known Conc 20 4.25ng/ml 0.225 5.29% Known Conc 20 13.2ng/ml 0.553 4.2% Inter-assay Sample n Mean SD CV% Known Conc 10 1.37ng/ml 0.06 4.71% Known Conc 10 4.8ng/ml 0.34 7.13% Known Conc 10 13.3ng/ml 0.75 5.65%
Sample typePlasma, Other biological fluids
Range0.05 ng/ml - 50 ng/ml
Sample specific recovery Sample type Average % Range Spike 99 93% - 107%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Does not react with: Sheep, Rabbit, Horse, Dog, Human
Abcam’s Tissue Plasminogen Activator (TPA) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active tissue Plasminogen Activator (TPA) in rat plasma and other biological fluids.
Functionally active rat TPA will form a covalent complex with the biotinylated Human PAI-1, which is bound to the avidin on the plate. Only free active TPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-TPA primary antibody binds to the captured TPA. Excess antibody is washed away and bound primary antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active TPA in the sample.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X TBS buffer pH 7.4 1 x 5ml 10X Wash Buffer 1 x 50ml HRP secondary antibody 1 x 10µl Anti-TPA primary antibody 1 vial Avidin pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests Biotinylated Human PAI-1 1 vial Rat TPA activity standard 1 vial TMB Substrate Solution 1 x 10ml
- Clade E
- Endothelial plasminogen activator inhibitor
ab198510 has been referenced in 1 publication.
- Ito Y et al. Generation and characterization of tissue-type plasminogen activator transgenic rats. J Thromb Thrombolysis 45:77-87 (2018). PubMed: 29168147