Overview

  • Product name

    Rat TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 25 pg/ml
  • Range

    82.3 pg/ml - 20000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.54 89% - 108%
    Serum 95.43 84% - 104%
    Plasma 93.79 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s TNF alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Rat TNF alpha  (lyophilized)  2 vials
    Recombinant Rat TNF alpha Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TNF alpha Microplate (12 x 8 wells) 1 unit
  • Research areas

  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily member 2
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor (TNF superfamily member 2)
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (duplicates +/- SD).

  • Representative Standard Curve using ab100784.

  • Representative Standard Curve using ab100784.

Protocols

References

This product has been referenced in:

  • Liu Z  et al. Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats. Evid Based Complement Alternat Med 2018:3674145 (2018). Read more (PubMed: 29541141) »
  • Akinrinmade FJ  et al. Changes in serum cytokine levels, hepatic and intestinal morphology in aflatoxin B1-induced injury: modulatory roles of melatonin and flavonoid-rich fractions from Chromolena odorata. Mycotoxin Res 32:53-60 (2016). Read more (PubMed: 26798045) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Merci beaucoup de nous avoir fait parvenir le dossier.

Je suis désolée que vous avez eu ce problème avec ce kit. Nous avons pas reçu aucun autre feedback négatif, ni sommes nous au courant d'un éventuel problème avec ce lot. Comme vous avezpar contre fait le contrôle avec l'autre kit en parallèle, il semble clair que il y avait un problème avec le kit. Nous allons donc vous le remplacer. Je tiens juste quand-même à vous rappeler que même si le kit devrait être stable pour plusieurs mois à 4°C, notre garantie est normalement de 6 mois. Merci pour votre compréhension.

Vous ne devez pas nous renvoyer les 4 strips. Je vais mettre en place l'envoie d'un kit de remplacement gratuit de ab100784. Nous allons l'envoyer à la même adresse que le kit original. Le numéro de commande est le xx et vous recevrez sous peu une confirmation de la commande. Veuillez s'il vous plaît ne pas hésiter àme contacter - de préférence aujourd'hui -si nous devons changer l'adresse de livraison ou si vous souhaitez un remboursement à la place du kit de remplacement.

Merci de votre coopération. Je suis à votre disposition pour toute autre question.

Je vous souhaite un agréable après-midi.

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Question
Answer

Merci de nous avoir informé du problème avec ce kit ab100784.

Comme discuté au téléphone, veuillez s'il vous plaît nous envoyer les données obtenues. Nous allons par la suite vous remplacer ce kit avec un nouveau.

Merci de votre coopération. Je me réjouis de recevoir votre réponse.

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