Rat TNF alpha ELISA Kit (ab46070)
- Datasheet
- Specific References (18)
- Protocols
Overview
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Product nameRat TNF alpha ELISA Kit
See all TNF alpha kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% A 12 543.5pg/ml 44.7 8.2% B 10 130.1pg/ml 10.3 7.9% Inter-assay Sample n Mean SD CV% A 9 541.4pg/ml 33.2 6.1% B 9 149.4pg/ml 9.8 6.5% -
Sample typeCell culture supernatant, Serum
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Assay typeSandwich (quantitative)
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Sensitivity< 15 pg/ml
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Range31.25 pg/ml - 1000 pg/ml
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Assay time3h 45m
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Rat
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Product overview
Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant rat TNF alpha
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-TNF alpha Red 1 x 400µl 2 x 400µl TMB Substrate 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl TNF alpha Microplate (12 x 8 well strips) 1 unit 2 units TNF alpha Standard (Lyophilized) Yellow 2 vials 4 vials -
Research areas
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FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Alternative names
- APC1 protein
- Cachectin
- DIF
see all -
Database links
- Entrez Gene: 24835 Rat
- SwissProt: P16599 Rat
- Unigene: 2275 Rat
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab46070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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ELISA: TNF alpha Rat ELISA Kit (ab46070)Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA ((PMA) ab120297) or PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (LPS+PMA) for the last 6 hours (duplicates +/- SD).
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ELISA: TNF alpha Rat ELISA Kit (ab46070)Standard curve with background signal subtracted (duplicates; +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab46070
Protocols
References
This product has been referenced in:
- Jiang P et al. Astragaloside IV Prevents Obesity-Associated Hypertension by Improving Pro-Inflammatory Reaction and Leptin Resistance. Mol Cells 41:244-255 (2018). Read more (PubMed: 29562733) »
- Gu R et al. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis. Front Pharmacol 9:331 (2018). Read more (PubMed: 29681857) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"