Key features and details
- Range: 0.01 ng/ml - 10 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Rat
Product nameRat uPA ELISA kit (total uPA antigen)
See all uPA kits
Sample typeUrine, Serum, Tissue Extracts, Cell culture media, EDTA Plasma, Cit plasma
Range0.01 ng/ml - 10 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Rat uPA ELISA kit (total uPA antigen) (ab272781) is intended for the quantitative determination of total urokinase plasminogen activator in rat plasma, serum, urine, cell culture media, or tissue extracts.
Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Rabbit Streptavidin-HRP Secondary Reagent 1 vial Anti-Rat uPA Primary Antibody Lyophilized Vial 1 vial Rat uPA ELISA Plate 1 unit Rat uPA Standard Lyophilized Vail 1 vial TMB Substrate 1 x 10ml
RelevanceUrokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).
- EC 188.8.131.52
ab272781 has not yet been referenced specifically in any publications.