Key features and details
- Rabbit polyclonal to RAVER1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAVER1 antibody
DescriptionRabbit polyclonal to RAVER1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Chinese hamster
Recombinant fragment, corresponding to a region between amino acids 556-606 of Human RAVER1 (UniProt ID: Q8IY67.1).
- 293T, HeLa and Jurkat whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 64 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus. Cytoplasm. Nuclear, in perinucleolar structures. Shuttles between nucleus and cytoplasm. Cytoplasm, at focal contacts and cell-cell contacts. Associated with myotubes during muscle differentiation.
- Information by UniProt
- KIAA1978 antibody
- Protein raver-1 antibody
- RAVER1 antibody
All lanes : Anti-RAVER1 antibody (ab157131) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutes
Immunoprecipitation with ab157131 at 6µg/mg lysate. 1 mg of 293T whole cell lysate used for IP (20% of IP loaded). Western blot labelling RAVER1 with ab157131 at 1 µg/ml. Detected by chemiluminescence with exposure time of 30 seconds.
ab157131 has not yet been referenced specifically in any publications.