Product nameAnti-RAVER1 antibody
DescriptionRabbit polyclonal to RAVER1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Chinese hamster
Recombinant fragment, corresponding to a region between amino acids 556-606 of Human RAVER1 (UniProt ID: Q8IY67.1).
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 64 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus. Cytoplasm. Nuclear, in perinucleolar structures. Shuttles between nucleus and cytoplasm. Cytoplasm, at focal contacts and cell-cell contacts. Associated with myotubes during muscle differentiation.
- Information by UniProt
- KIAA1978 antibody
- Protein raver-1 antibody
- RAVER1 antibody
All lanes : Anti-RAVER1 antibody (ab157131) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3 minutes
Immunoprecipitation with ab157131 at 6µg/mg lysate. 1 mg of 293T whole cell lysate used for IP (20% of IP loaded). Western blot labelling RAVER1 with ab157131 at 1 µg/ml. Detected by chemiluminescence with exposure time of 30 seconds.
ab157131 has not yet been referenced specifically in any publications.