Product nameAnti-RAVER2 antibody [EPR12601]
See all RAVER2 primary antibodies
DescriptionRabbit monoclonal [EPR12601] to RAVER2
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human RAVER2.
Database link: Q9HCJ3
- Human fetal lung, HT1080, 293T, Human fetal brain, and Jurkat lysates; Human cervical carcinoma and tonsil tissues; 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 74 kDa.|
FunctionMay bind single-stranded nucleic acids.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus. Cytoplasm. May shuttle between the nucleus and the cytoplasm.
- Information by UniProt
- DKFZp762D1011 antibody
- FLJ10770 antibody
- KIAA1579 antibody
All lanes : Anti-RAVER2 antibody [EPR12601] (ab174321) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : HT1080 cell lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lane 5 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Immunofluorescent analysis of 293T cells labeling RAVER2 with ab174321 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RAVER2 with ab174321 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling RAVER2 with ab174321 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from fetal lung lysate, immunoprecipitated using ab174321 at 1/10 dilution.
This product has been referenced in:
- Liu H et al. Alternative splicing analysis in human monocytes and macrophages reveals MBNL1 as major regulator. Nucleic Acids Res 46:6069-6086 (2018). Read more (PubMed: 29771377) »
- Bartoletti-Stella A et al. Messenger RNA processing is altered in autosomal dominant leukodystrophy. Hum Mol Genet 24:2746-56 (2015). WB ; Mouse . Read more (PubMed: 25637521) »