Recombinant
RabMAb

Anti-RAVER2 antibody [EPR12601] (ab174321)

Overview

  • Product name
    Anti-RAVER2 antibody [EPR12601]
    See all RAVER2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12601] to RAVER2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RAVER2.
    Database link: Q9HCJ3

  • Positive control
    • Human fetal lung, HT1080, 293T, Human fetal brain, and Jurkat lysates; Human cervical carcinoma and tonsil tissues; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      May bind single-stranded nucleic acids.
    • Sequence similarities
      Contains 3 RRM (RNA recognition motif) domains.
    • Cellular localization
      Nucleus. Cytoplasm. May shuttle between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp762D1011 antibody
      • FLJ10770 antibody
      • KIAA1579 antibody
      • Protein raver 2 antibody
      • Protein raver-2 antibody
      • Raver2 antibody
      • RAVR2_HUMAN antibody
      • Ribonucleoprotein PTB binding 2 antibody
      • Ribonucleoprotein PTB-binding 2 antibody
      see all

    Images

    • All lanes : Anti-RAVER2 antibody [EPR12601] (ab174321) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : HT1080 cell lysate
      Lane 3 : 293T lysate
      Lane 4 : Human fetal brain lysate
      Lane 5 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 74 kDa

    • Immunofluorescent analysis of 293T cells labeling RAVER2 with ab174321 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RAVER2 with ab174321 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling RAVER2 with ab174321 at 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from fetal lung lysate, immunoprecipitated using ab174321 at 1/10 dilution.

    References

    This product has been referenced in:
    • Bartoletti-Stella A  et al. Messenger RNA processing is altered in autosomal dominant leukodystrophy. Hum Mol Genet 24:2746-56 (2015). WB ; Mouse . Read more (PubMed: 25637521) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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