Overview

  • Product name
    RAW 264.7 whole cell lysate
  • General notes
    Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor).
    Growth media: DMEM and 10% fetal bovine serum.


    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Lysate notes
    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas
  • Background
    Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.

Applications

Our Abpromise guarantee covers the use of ab7187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Ready to load.

References

This product has been referenced in:
  • Zaman RT  et al. Imaging cellular pharmacokinetics of 18F-FDG and 6-NBDG uptake by inflammatory and stem cells. PLoS One 13:e0192662 (2018). Read more (PubMed: 29462173) »
  • Madrigal-Matute J  et al. TWEAK/Fn14 interaction promotes oxidative stress through NADPH oxidase activation in macrophages. Cardiovasc Res 108:139-47 (2015). Read more (PubMed: 26224570) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells.

https://www.abcam.com/index.html?datasheet=52174&tab=abreviews&intabreviewid=13947

In his treatment, this customer used oxytocin, which we carry as ab120186 and phorbol 12-myristate 13-acetate (PMA), which we carry as ab120297.

https://www.abcam.com/Oxytocin-ab120186.html

https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-ab120297.html

I do not know enough about the protein to tell you definitively whether or not this protein is endogenously phosphorylated in C2C12 cells. I do have an image of ab52174 used in WB with untreated RAW264.7 cells though, which we sell as ab7187. I'll be sure to get that WB image added to the datasheet online, but it will take a few days to appear.

https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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