Overview

  • Product name

  • Description

    Rabbit polyclonal to RB-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RB-1 aa 446-476 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: NP_694453.2

  • Positive control

    • NCI-H460 cell line lysate
  • General notes

     This product was previously labelled as RBM45

     

Properties

Applications

Our Abpromise guarantee covers the use of ab123912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.

Target

  • Function

    RNA-binding protein with binding specificity for poly(C). May play an important role in neural development.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic. May shuttle between cytoplasm and nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Developmentally-regulated RNA-binding protein 1 antibody
    • DRB1 antibody
    • FLJ44612 antibody
    • MGC42237 antibody
    • Putative RNA binding protein RB 1 antibody
    • RB-1 antibody
    • RBM45 antibody
    • RBM45_HUMAN antibody
    • RNA-binding motif protein 45 antibody
    • RNA-binding protein 45 antibody
    see all

Images

  • Anti-RB-1 antibody (ab123912) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 53 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK-293)
Permeabilization
Yes - 0.3% Triton X-100 in Blocking Buffer
Specification
HEK-293
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 22 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 02 2016

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