Anti-RB-1 antibody (ab224426)
Key features and details
- Rabbit polyclonal to RB-1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RB-1 antibody -
Description
Rabbit polyclonal to RB-1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human RB-1 aa 1-150.
Database link: Q8IUH3 -
Positive control
- ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
RNA-binding protein with binding specificity for poly(C). May play an important role in neural development. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. May shuttle between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 129831 Human
- Entrez Gene: 241490 Mouse
- Entrez Gene: 266631 Rat
- Omim: 608888 Human
- SwissProt: Q8IUH3 Human
- SwissProt: Q8BHN5 Mouse
- SwissProt: Q8CFD1 Rat
- Unigene: 377257 Human
see all -
Alternative names
- Developmentally-regulated RNA-binding protein 1 antibody
- DRB1 antibody
- FLJ44612 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab224426 has been referenced in 2 publications.
- Liu Y et al. miR-222-5p promotes dysfunction of human vascular smooth muscle cells by targeting RB1. Environ Toxicol 37:683-694 (2022). PubMed: 34862716
- Yuan XW et al. Effect of miR-515-5p on Proliferation and Drug Sensitivity of Retinoblastoma Cells. Cancer Manag Res 12:12087-12098 (2020). PubMed: 33262657