• Product name
    Anti-Rb antibody [Rb1 1F8]
    See all Rb primary antibodies
  • Description
    Mouse monoclonal [Rb1 1F8] to Rb
  • Host species
  • Specificity
    This antibody reacts with hyperphosphorylated and un (under) phosphorylated forms of Rb protein.
  • Tested applications
    Suitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Chimpanzee
  • Immunogen

    Fusion protein. Rb-b-galactosidase fusion protein spanning nucleotides 1126-1973 of human Rb gel purified from bacterial lysates (R. Grand et al. 1989).

  • Epitope
    The epitope has been mapped between aa 703-772 of human RB1.
  • Positive control
    • Tested in a panel of human cell lines and CV-1 cell line (established from monkey kidney epithelium).



Our Abpromise guarantee covers the use of ab24 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • Function
    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
  • Tissue specificity
    Expressed in the retina.
  • Involvement in disease
    Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma
  • Sequence similarities
    Belongs to the retinoblastoma protein (RB) family.
  • Domain
    The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
  • Post-translational
    Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
    • GOS563 exon 17 substitution mutation causes premature stop antibody
    • OSRC antibody
    • Osteosarcoma antibody
    • p105-Rb antibody
    • P105RB antibody
    • PP105 antibody
    • pp110 antibody
    • PPP1R130 antibody
    • pRb antibody
    • Prepro retinoblastoma associated protein antibody
    • Protein phosphatase 1 regulatory subunit 130 antibody
    • Rb antibody
    • RB transcriptional corepressor 1 antibody
    • RB_HUMAN antibody
    • RB1 antibody
    • RB1 gene antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma suspectibility protein antibody
    • Retinoblastoma-associated protein antibody
    see all


  • Lysates prepared from lung and kidney of VerUTR transgenic and wildtype mice were analyzed by Western Blot probed with ab24.

    Increased expression of Rb1 was detected in the organs from the VerUTR transgenic mice. Cells were seeded onto 6-well plates at 2×105 cells per well overnight. They were then transfected with 1 µg of VerUTR or control vector in combination with scrambled RNA or siRNA against VerUTR. Proteins were extracted 48 hours after transfection by lysing in 60 µl of lysis buffer containing protease inhibitors (150 mM NaCl, 25 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, 1% Triton X-100, 8 M Urea, and 1x protease inhibitor cocktail). Tissues were disrupted in appropriate volume of lysis buffer depending on tissue weight. All samples were subjected to SDS-PAGE and then transferred to nitrocellulose membranes followed by incubating with ab24 at 1/500 dilution at 4°C overnight. The secondary antibody used was goat anti-mouse IgG at 1/2000 dilution at room temperature.

  • Anti-Rb antibody [Rb1 1F8] (ab24) at 1/1000 dilution + Human fibroblast nuclear cell lysate

    Mouse polyclonal-HRP conjugated

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 105 kDa
    Observed band size: 100-150 kDa
    why is the actual band size different from the predicted?

    Exposure time: 1 minute

    Primary antibody was incubated for 16 hours.

    Blocked with 5% milk for 1 hour.

    See Abreview


This product has been referenced in:
  • Wangyang Y  et al. MiR-199a-3p inhibits proliferation and induces apoptosis in rheumatoid arthritis fibroblast-like synoviocytes via suppressing retinoblastoma 1. Biosci Rep 38:N/A (2018). Read more (PubMed: 30352835) »
  • Gong Y  et al. Circ-ZEB1.33 promotes the proliferation of human HCC by sponging miR-200a-3p and upregulating CDK6. Cancer Cell Int 18:116 (2018). Read more (PubMed: 30123094) »
See all 21 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 08 2012

Western blot
Human Cell lysate - whole cell (lung and foreskin fibroblast cells; MOUSE lung)
Loading amount
30 µg
lung and foreskin fibroblast cells; MOUSE lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Apr 10 2006

Western blot
Human Cell lysate - whole cell (Hela)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mrs. Bernadette Ferraro

Verified customer

Submitted Dec 19 2005


Thank you for your enquiry. Ab24 is predicted to react with rat Rb (97% identity with immunogen) due to sequence homology but this has not been specifically tested yet. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

Read More


Thank you for your enquiry. Ab24 also recognises the phosphorylates and unphospohorylated forms of Rb1, so it is nonspecific. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Read More
Western blot
Human Cell lysate - nuclear (human fibroblast)
human fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 05 2005

For licensing inquiries, please contact partnerships@abcam.com

Sign up