Product nameAnti-Rb (methyl K860) antibody
See all Rb primary antibodies
DescriptionRabbit polyclonal to Rb (methyl K860)
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Rb conjugated to keyhole limpet haemocyanin. Methylated and acetylated synthetic peptide surrounding lysine 860
Database link: P06400
- WB: MOLT-4 whole cell lysate. Dot blot: Retinoblastoma [Monomethyl Lys860] peptide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab263969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use a concentration of 0.25 - 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa.|
FunctionKey regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Tissue specificityExpressed in the retina.
Involvement in diseaseChildhood cancer retinoblastoma
Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
DomainThe Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
modificationsPhosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
- Information by UniProt
- Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
- GOS563 exon 17 substitution mutation causes premature stop antibody
- OSRC antibody
Anti-Rb (methyl K860) antibody (ab263969) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 35 µg
Rabbit secondary HRP at 1/70000 dilution
Predicted band size: 106 kDa
Primary incubation overnight at 4°C.
Secondary incubation for 45 minutes at RT.
Dot blot analysis of Rb (methyl K860) labeled with ab263969 at the indicated concentrations.
1: Unmodified Rb peptide.
2: Rb [Monomethyl Lys860] peptide.
Primary incubation overnight at 4°C.
Secondary antibody is a Goat anti-Rabbit HRP conjugate at 1/40,000 dilution for 30 minutes at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263969 has not yet been referenced specifically in any publications.