RecombinantRabMAb

Recombinant Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)

Submit a review Submit a question References (1)
Western blot - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
  • Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10849] to Rb (phospho S608)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb (phospho S608) antibody [EPR10849]
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EPR10849] to Rb (phospho S608)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Rb aa 600-700 (phospho S608). The exact sequence is proprietary.
    Database link: P06400

  • Positive control

    • FBS treated Jurkat cell lysates; Human melanoma and retina tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab172975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 106 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

    • Tissue specificity

      Expressed in the retina.

    • Involvement in disease

      Childhood cancer retinoblastoma
      Bladder cancer
      Osteogenic sarcoma

    • Sequence similarities

      Belongs to the retinoblastoma protein (RB) family.

    • Domain

      The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.

    • Post-translational
      modifications

      Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
      N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
      Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
        • GOS563 exon 17 substitution mutation causes premature stop antibody
        • OSRC antibody
        • Osteosarcoma antibody
        • p105-Rb antibody
        • P105RB antibody
        • PP105 antibody
        • pp110 antibody
        • PPP1R130 antibody
        • pRb antibody
        • Prepro retinoblastoma associated protein antibody
        • Protein phosphatase 1 regulatory subunit 130 antibody
        • Rb antibody
        • RB transcriptional corepressor 1 antibody
        • RB_HUMAN antibody
        • RB1 antibody
        • RB1 gene antibody
        • Retinoblastoma 1 antibody
        • Retinoblastoma suspectibility protein antibody
        • Retinoblastoma-associated protein antibody
        see all

      Images

      • Western blot - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
        Western blot - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
        All lanes : Anti-Rb (phospho S608) antibody [EPR10849] (ab172975) at 1/10000 dilution

        Lane 1 : Jurkat cell lysate, untreated
        Lane 2 : Jurkat cell lysate, treated with FBS

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 106 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)

        Immunohistocemical analysis of paraffin-embedded Human retina tissue labeling Rb (phospho S608) with ab172975 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)

        Immunohistocemical analysis of paraffin-embedded Human melanoma tissue labeling Rb (phospho S608) with ab172975 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)
        Anti-Rb (phospho S608) antibody [EPR10849] (ab172975)

      References (1)

      Publishing research using ab172975? Please let us know so that we can cite the reference in this datasheet.

      ab172975 has been referenced in 1 publication.

      • Sheldon LA Inhibition of E2F1 activity and cell cycle progression by arsenic via retinoblastoma protein. Cell Cycle 16:2058-2072 (2017). PubMed: 28880708

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab172975.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com