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    rb-phospho-s780-antibody-ab47763.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Rb
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Anti-Rb (phospho S780) antibody (ab47763)

  • Datasheet
Reviews (2)Q&A (1)References (29)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab47763)
  • Western blot - Anti-Rb (phospho S780) antibody (ab47763)
  • Western blot - Anti-Rb (phospho S780) antibody (ab47763)

Key features and details

  • Rabbit polyclonal to Rb (phospho S780)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Rb (phospho S780) antibody
    See all Rb primary antibodies
  • Description

    Rabbit polyclonal to Rb (phospho S780)
  • Host species

    Rabbit
  • Specificity

    This antibody detects Rb only when phosphorylated at Serine 780.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Rb (phospho S780).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    PBS Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human Rb protein (ab83205)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (1)
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 106 kDa).
IHC-P
1/50 - 1/100.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 106 kDa).
IHC-P
1/50 - 1/100.

Target

  • Function

    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
  • Tissue specificity

    Expressed in the retina.
  • Involvement in disease

    Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma
  • Sequence similarities

    Belongs to the retinoblastoma protein (RB) family.
  • Domain

    The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
  • Post-translational
    modifications

    Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P06400 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5925 Human
    • Omim: 614041 Human
    • SwissProt: P06400 Human
    • Unigene: 408528 Human
    • Alternative names

      • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
      • GOS563 exon 17 substitution mutation causes premature stop antibody
      • OSRC antibody
      • Osteosarcoma antibody
      • p105-Rb antibody
      • P105RB antibody
      • PP105 antibody
      • pp110 antibody
      • PPP1R130 antibody
      • pRb antibody
      • Prepro retinoblastoma associated protein antibody
      • Protein phosphatase 1 regulatory subunit 130 antibody
      • Rb antibody
      • RB transcriptional corepressor 1 antibody
      • RB_HUMAN antibody
      • RB1 antibody
      • RB1 gene antibody
      • Retinoblastoma 1 antibody
      • Retinoblastoma suspectibility protein antibody
      • Retinoblastoma-associated protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab47763)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab47763)

      ab47763 at 1/50 dilution staining paraffin-embedded human breast carcinoma; left without and right with immunising peptide.

    • Western blot - Anti-Rb (phospho S780) antibody (ab47763)
      Western blot - Anti-Rb (phospho S780) antibody (ab47763)
      All lanes : Anti-Rb (phospho S780) antibody (ab47763) at 1/500 dilution

      Lane 1 : K562 cell extract treated with serum for 20 hours
      Lane 2 : K562 cell extract

      Predicted band size: 106 kDa



      Typically 5-30ug of total protein was loaded per lane of the gel.
    • Western blot - Anti-Rb (phospho S780) antibody (ab47763)
      Western blot - Anti-Rb (phospho S780) antibody (ab47763)

      Western blot analysis of extracts from K562 cells treated or untreated with serum using Rb antibody and ab47763.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (29)

    Publishing research using ab47763? Please let us know so that we can cite the reference in this datasheet.

    ab47763 has been referenced in 29 publications.

    • Tu Q  et al. A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal. Dis Model Mech 12:N/A (2019). PubMed: 30910991
    • Huang D  et al. PIK3CA mutations contribute to fulvestrant resistance in ER-positive breast cancer. Am J Transl Res 11:6055-6065 (2019). PubMed: 31632573
    • Butler MB  et al. Rho kinase-dependent apical constriction counteracts M-phase apical expansion to enable mouse neural tube closure. J Cell Sci 132:N/A (2019). PubMed: 31182644
    • Zhang H & Hu N Telomerase reverse transcriptase induced thyroid carcinoma cell proliferation through PTEN/AKT signaling pathway. Mol Med Rep 18:1345-1352 (2018). PubMed: 29901196
    • Ohmine S  et al. Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells. Oncol Rep 39:1725-1730 (2018). PubMed: 29393405
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Rb (phospho S780) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (Neuroepithelium and surface ectoderm)
    Specification
    Neuroepithelium and surface ectoderm
    Read More

    Dr. Gabriel Galea

    Verified customer

    Submitted Jan 21 2020

    Question

    High background in human cell line western blot.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Thank you for calling Abcam earlier today.
    I am sorry about the issues you were having with ab47763 in western blotting. As we discussed, I am sending you an aliquot of ab32513 as a free of charge replacement. Your new order number is *.
    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Western blot abreview for Anti-Rb (phospho S780) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF-7)
    Loading amount
    5 µg
    Specification
    MCF-7
    Treatment
    CDK7 inhibitor
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Dr. Dean Heathcote

    Verified customer

    Submitted Oct 28 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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