Immunocytochemistry/ Immunofluorescence abreview for Anti-Rb (phospho T821) antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Sample
Mouse Cell (B cell line)
Specification
B cell line
Permeabilization
Yes - 0,1% TritonX-100/PBS for 10 minutes at RT
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Serum / 3% BSA in TBS-T
Dilution
1/300

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor­ 546 Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 546
Dilution
1/500

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Submitted Jun 03 2014

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