Key features and details
- Mouse monoclonal [2C8A8] to Rb2 p130
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Rb2 p130 antibody [2C8A8]
See all Rb2 p130 primary antibodies
DescriptionMouse monoclonal [2C8A8] to Rb2 p130
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human Rb2 p130 aa 939-1139. (Purified from E.coli).
RNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPT RLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYP FVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREI NSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGS H
Database link: Q08999
- Human Rb2 p130 (aa939-1139) recombinant protein; Rb2 p130 (aa939-1139)-hIgGFc transfected HEK293 cell lysate; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab201943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 128 kDa.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceTwo retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
- 130 kDa retinoblastoma associated protein antibody
- FLJ26459 antibody
- p130 antibody
Anti-Rb2 p130 antibody [2C8A8] (ab201943) at 1/500 dilution + Human Rb2 p130 (aa939-1139) recombinant protein
Predicted band size: 128 kDa
Expected MWt is 48.7 kDa.
All lanes : Anti-Rb2 p130 antibody [2C8A8] (ab201943) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Rb2 p130 (aa939-1139)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 128 kDa
Flow cytometric analysis of HeLa cells labeling Rb2 p130 with ab201943 at 1/200 dilution (green) compared to a negative control (red).
ab201943 has not yet been referenced specifically in any publications.