Overview

  • Product name

  • Description

    Rabbit polyclonal to Rb2 p130
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    KYPQEEQPRQQRGRK

    , corresponding to amino acids 175-189 of Human Rb2 p130

  • Positive control

    • HeLa and Jurkat T cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

Target

  • Relevance

    Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • 130 kDa retinoblastoma associated protein antibody
    • FLJ26459 antibody
    • p130 antibody
    • PRB 2 antibody
    • PRB2 antibody
    • Rb 2 antibody
    • Rb2 antibody
    • RBL 2 antibody
    • RBL2 antibody
    • RBR 2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma like protein 2 antibody
    • Retinoblastoma related gene antibody
    see all

Images

  • All lanes : Anti-Rb2 p130 antibody (ab71143) at 1/2000 dilution

    Lane 1 : Jurkat T cell lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 128 kDa
    Observed band size: 128 kDa

References

This product has been referenced in:

  • Starrett GJ  et al. Polyomavirus T Antigen Induces APOBEC3B Expression Using an LXCXE-Dependent and TP53-Independent Mechanism. MBio 10:N/A (2019). Read more (PubMed: 30723127) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19 cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Specification
ARPE-19 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 18 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Vero cell)
Permeabilization
Yes - 0.25% Tx-100
Specification
Vero cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 29 2017

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