Recombinant
RabMAb

Recombinant Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Overview

  • Product name
    Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]
    See all Rb2 p130 primary antibodies
  • Description
    Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    corresponding to Human Rb2 p130.

  • Positive control
    • WB: Jurkat and RAW 264.7 cell lysate; mouse heart tissue lysate IHC-P: human breast and lung carcinoma tissue, Mouse cerebrum
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab68136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

For unpurified use at 1/10000 - 1/500000.

IHC-P 1/600.

 See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

 

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Relevance
      Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • 130 kDa retinoblastoma associated protein antibody
      • FLJ26459 antibody
      • p130 antibody
      • PRB 2 antibody
      • PRB2 antibody
      • Rb 2 antibody
      • Rb2 antibody
      • RBL 2 antibody
      • RBL2 antibody
      • RBR 2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma like protein 2 antibody
      • Retinoblastoma related gene antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution (Purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase
      Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 128 kDa
      Observed band size: 128 kDa

    • All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/5000 dilution (Purified)

      Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates. Then the membrane was incubated with phosphatase
      Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 128 kDa
      Observed band size: 128 kDa

    • All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution (Purified)

      Lane 1 : Mouse heart lysates. Then the membrane was incubated with phosphatase
      Lane 2 : Mouse heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 128 kDa
      Observed band size: 128 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/500000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labeled goat anti-mouse at 1/2000 dilution

      Predicted band size: 128 kDa
      Observed band size: 128 kDa

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68136 (unpurified) at a dilution of 1/100.

    References

    This product has been referenced in:
    • Divakar SK  et al. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia 30:86-93 (2016). Read more (PubMed: 26174628) »
    • Orlando S  et al. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res 43:6860-73 (2015). Read more (PubMed: 26071952) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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