Overview

  • Product name
  • Description
    Rabbit polyclonal to RbAp46
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ICC, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
    Predicted to work with: Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human RbAp46 aa 1-13.
    Sequence:

    MASKEMFEDTVEEC


    (Peptide available as ab5013)

Properties

Applications

Our Abpromise guarantee covers the use of ab3535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22223663
ICC Use a concentration of 3.33 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47.6 kDa).Can be blocked with RbAp46 peptide (ab5013).
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similarities
    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • G1/S transition control protein binding protein RbAp46 antibody
    • Histone acetyltransferase type B subunit 2 antibody
    • Histone binding protein RBBP7 antibody
    • Histone-binding protein RBBP7 antibody
    • MGC138867 antibody
    • MGC138868 antibody
    • Nucleosome remodeling factor subunit RBAP46 antibody
    • Nucleosome-remodeling factor subunit RBAP46 antibody
    • RBAP46 antibody
    • RBBP 7 antibody
    • RBBP-7 antibody
    • RBBP7 antibody
    • RBBP7_HUMAN antibody
    • Retinoblastoma binding protein 7 antibody
    • Retinoblastoma binding protein p46 antibody
    • Retinoblastoma-binding protein 7 antibody
    • Retinoblastoma-binding protein p46 antibody
    • Retinoblastoma-binding protein RbAp46 antibody
    see all

Images

  • ab3535 staining RbAp46 in Hela cells by Immunocytochemistry/ immunofluorescence. 

  • All lanes : Anti-RbAp46 antibody (ab3535) at 0.5 µg/ml

    Lane 1 : Cell extract prepared from DU-145 2 cells
    Lane 2 : Cell extract prepared from LNCaP cells
    Lane 3 : Cell extract prepared from HepG2 cells

    Predicted band size: 47.6 kDa



    Western blot of RbAp46 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3535.
  • Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.

References

This product has been referenced in:
  • Zhang N  et al. High expression of EZH2 as a marker for the differential diagnosis of malignant and benign myogenic tumors. Sci Rep 8:12331 (2018). Read more (PubMed: 30120321) »
  • Hoffmeister H  et al. CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. Nucleic Acids Res 45:10534-10554 (2017). Read more (PubMed: 28977666) »
See all 10 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you very much for your interest in ab488. I am not sure whether you are interested in receiving more information about our testing program or if you are ready to receive the discount code, so I am sending all of the information about the program just in case you are still deciding.
Since ab488 has not been tested with chicken samples, I can offer a discount off a future purchase if you buy this antibody now, test it in chicken and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test this antibody. I will then send a discount code.
2. Purchase ab488 either by phone, fax, or online (www.abcam.com).
3. Test it in chicken.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated!
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SW480, colorectal cancer)
Loading amount
30 µg
Specification
SW480, colorectal cancer
Gel Running Conditions
Reduced Denaturing (12%/4%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 12 2008

Answer

Thank you for your patience. The originator of ab3535 has said that the antibody should not cross react with the RbAP48 protein. Please contact us again if you have any additional questions.

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Answer

Thank you for your enquiry. For ab86 and ab488, the antibodies have not been tested against RbAp46 so we do not know whether or not the antibodies would react with it. I'm still waiting for information on ab3535 and will let you know when I receive.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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