• Product name
  • Description
    Rabbit polyclonal to RbAp48
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 406-425 of Human and Mouse CAF 1 (p48).

  • General notes
    Locus link: 350005 Alternative names: similar to chromatin assembly factor 1 p48 subunit

    A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).



Our Abpromise guarantee covers the use of ab1765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 20 µg/ml.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa).


  • Function
    Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similarities
    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF I p48 antibody
    • CAF-1 subunit C antibody
    • CAF-I 48 kDa subunit antibody
    • CAF-I p48 antibody
    • Chromatin assembly factor 1 subunit C antibody
    • Chromatin assembly factor I p48 subunit antibody
    • Chromatin assembly factor/CAF 1 p48 subunit antibody
    • Histone-binding protein RBBP4 antibody
    • MSI1 protein homolog antibody
    • Nucleosome-remodeling factor subunit RBAP48 antibody
    • NURF55 antibody
    • RB binding protein 4 chromatin remodeling factor antibody
    • RbAp 48 antibody
    • RBAP48 antibody
    • RBBP-4 antibody
    • RBBP4 antibody
    • RBBP4_HUMAN antibody
    • Retinoblastoma binding protein 4 antibody
    • Retinoblastoma binding protein p48 antibody
    • Retinoblastoma-binding protein 4 antibody
    • Retinoblastoma-binding protein p48 antibody
    see all


  • ab1765 staining RbAp48 in MDCK cell pellets by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Pellets were fixed with paraformaldehyde and blocked with 1% BSA for 3 hours at 22°C; antigen retrieval was by heat mediation in a citrate buffer, permeabilization was with Tween-20. Samples were incubated with primary antibody (1/100 in TBST) for 16 hours. A HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Rabbit polyclonal to CAF 1 p48 (ab1765)
    Each lane contains 20 ug of HeLa whole cell lysate.

    Lane 1: ab1765 1/500
    Lane 2: ab1765 1/1000

    Secondary antibody - Goat anti-Rabbit (HRP) (ab6721) at 1/2000.

  • Anti-RbAp48 antibody (ab1765) at 1/1000 dilution
    Anti-rabbit IgG HRP

    Developed using the ECL technique.

    Predicted band size: 47.6 kDa
    Observed band size: 40,55 kDa (why is the actual band size different from the predicted?)

    Exposure time: 1 minute

    ab1765 detecting p48 CAF1 from human LCL whole cell lysates by Western Blot.

    See Abreview

  • ab1765 used in Immunoprecipitation of HeLa whole cell lysate.

    Whole cell extract (containing 100µg of soluble protein) was incubated with 20µg of antibody overnight. Protein A-Agarose was added to the immunocomplexes and incubated at room temperature for three hours. Immunocomplexes were eluted and then resolved using 7.5% SDS-PAGE under reducing conditions. For the detection of HDAC1, 2 and 3 a non-Abcam mouse monoclonal antibody was used at a dilution of 1/800. Results support the understanding that HDAC1 and 2 are in the same complex together with p48. Note that HDAC1 does not coprecipitate HDAC2, probably displaced by the antibody during the experiment.

    Lane 1: Input

    Lane 2: HDAC1 (ab1769)

    Lane 3: p48 (ab1765)

    Lane 4: No antibody control

    This image is courtesy of an Abreview by Hugh Spotswood submitted on 9 February 2006.

    See Abreview

  • ab1765 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview


This product has been referenced in:
  • Sun D  et al. LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination. EMBO Rep N/A:N/A (2017). Read more (PubMed: 28808113) »
  • Bode D  et al. Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells. Mol Cell Proteomics 15:878-91 (2016). WB . Read more (PubMed: 26714524) »

See all 10 Publications for this product

Customer reviews and Q&As

This product has been tested in IF and would be gaurateed to work in that application.


Sign up