Product nameAnti-RbAp48 antibody
See all RbAp48 primary antibodies
DescriptionRabbit polyclonal to RbAp48
Tested applicationsSuitable for: ICC/IF, WB, ChIPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Zebrafish
- ab38135 gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab38135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 48 kDa).|
|ChIP||Use at an assay dependent concentration. PubMed: 25051498|
FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats.
- Information by UniProt
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
All lanes : Anti-RbAp48 antibody (ab38135) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 :
Jurkat whole cell lysate (ab7899)
Lane 4 :
A431 whole cell lysate (ab7909)
Lane 5 :
HEK293 whole cell lysate (ab7902)
Lane 6 :
HepG2 whole cell lysate (ab7900)
Lane 7 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab38135 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38135, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
This product has been referenced in:
- Muralidharan B et al. LHX2 Interacts with the NuRD Complex and Regulates Cortical Neuron Subtype Determinants Fezf2 and Sox11. J Neurosci 37:194-203 (2017). CHIPseq, IP ; Mouse . Read more (PubMed: 28053041) »
- Gu X et al. Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. J Biol Chem 289:14881-95 (2014). Read more (PubMed: 24695740) »