Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3412] to RbAp48
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-RbAp48 antibody [EPR3412]
See all RbAp48 primary antibodies
DescriptionRabbit monoclonal [EPR3412] to RbAp48
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human RbAp48 aa 400 to the C-terminus. The exact sequence is proprietary.
- HeLa, Jurkat, Raji and 293T cell lysates; Human breast carcinoma tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats.
- Information by UniProt
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
All lanes : Anti-RbAp48 antibody [EPR3412] (ab92344) at 1/20000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raji
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
ab92344 at 1/250 dilution staining RbAp48 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ab92344 has been referenced in 2 publications.
- Akiyama T et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res 22:307-18 (2015). PubMed: 26324425
- Davidovich C et al. Promiscuous RNA binding by Polycomb repressive complex 2. Nat Struct Mol Biol 20:1250-7 (2013). PubMed: 24077223