Recombinant Anti-RBBP4 antibody [EPR3412] - ChIP Grade - BSA and Azide free (ab247547)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3412] to RBBP4 - ChIP Grade – BSA and Azide free
- Suitable for: IHC-P, WB, ChIP-sequencing
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-RBBP4 antibody [EPR3412] - ChIP Grade - BSA and Azide free
See all RBBP4 primary antibodies -
Description
Rabbit monoclonal [EPR3412] to RBBP4 - ChIP Grade – BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB and IHC: HeLa, Jurkat, Raji and 293T cell lysates; Human breast carcinoma tissue. ChIP-Seq: Chromatin from HeLa cells.
-
General notes
ab247547 is the carrier-free version of ab92344.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3412 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
|
|
ChIP-sequencing |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
ChIP-sequencing
Use at an assay dependent concentration. |
Target
-
Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
Sequence similarities
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Omim: 602923 Human
- SwissProt: Q09028 Human
- SwissProt: Q60972 Mouse
- Unigene: 16003 Human
- Unigene: 12145 Mouse
-
Alternative names
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
see all
Images
-
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 µg of ab92344 [EPR3412]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here.
-
All lanes : Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344) at 1/20000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaThis data was developed using ab92344, the same antibody clone in a different buffer formulation.
-
This data was developed using ab92344, the same antibody clone in a different buffer formulation.
ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab247547 has not yet been referenced specifically in any publications.