Key features and details
- Rabbit polyclonal to RbBP5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RbBP5 antibody
See all RbBP5 primary antibodies
DescriptionRabbit polyclonal to RbBP5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Zebrafish
Synthetic peptide corresponding to Human RbBP5 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).|
FunctionAs part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesContains 6 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- RBBP 5 antibody
- RBBP-5 antibody
- RBBP5 antibody
All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 60,65 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
RbBP5 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed. The antibody should cross-react with both isoform 1 (59-kDa) and isoform 2 (55-kDa) of RbBP5.
ab80074 has been referenced in 1 publication.
- Fang L et al. SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Cancer Cell 34:103-118.e9 (2018). PubMed: 30008322