Overview

  • Product name

  • Description

    Rabbit polyclonal to RbBP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human RbBP5 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87828)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab80074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Contains 6 WD repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • RBBP 5 antibody
    • RBBP-5 antibody
    • RBBP5 antibody
    • RBBP5_HUMAN antibody
    • RBQ 3 antibody
    • RBQ3 antibody
    • Retinoblastoma binding protein 5 antibody
    • Retinoblastoma binding protein RBQ3 antibody
    • Retinoblastoma binding protein5 antibody
    • Retinoblastoma-binding protein 5 antibody
    • Retinoblastoma-binding protein RBQ-3 antibody
    • SWD1 antibody
    • SWD1, Set1c WD40 repeat protein, homolog antibody
    see all

Images

  • All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml

    Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 60,65 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    RbBP5 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed. The antibody should cross-react with both isoform 1 (59-kDa) and isoform 2 (55-kDa) of RbBP5.

References

This product has been referenced in:

  • Fang L  et al. SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Cancer Cell 34:103-118.e9 (2018). Read more (PubMed: 30008322) »
See 1 Publication for this product

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