Key features and details
- Mouse monoclonal [CL4289] to RBCK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-RBCK1 antibody [CL4289]
See all RBCK1 primary antibodies
DescriptionMouse monoclonal [CL4289] to RBCK1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
EpitopeBinds to an epitope located within the peptide sequence KDMVFLDYGFPPVLQ as determined by overlapping synthetic peptides.
- HepG2 cell lysate; Human duodenum, liver, kidney, endometrium and testis tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab219955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteoasome. Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates. LUBAC conjugates linear polyubiquitin to IKBKG at 'Lys-285' and 'Lys-309' and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Sequence similaritiesContains 1 B box-type zinc finger.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 ubiquitin-like domain.
modificationsAuto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome.
Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity.
- Information by UniProt
- C20orf18 antibody
- Chromosome 20 open reading frame 18 antibody
- HBV associated factor 4 antibody
Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling RBCK1 with ab219955 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling RBCK1 with ab219955 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling RBCK1 with ab219955 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling RBCK1 with ab219955 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling RBCK1 with ab219955 at 1/50 dilution.
Anti-RBCK1 antibody [CL4289] (ab219955) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 58 kDa
ab219955 has not yet been referenced specifically in any publications.