Anti-RBM10/S1-1 antibody (ab72423)
Key features and details
- Rabbit polyclonal to RBM10/S1-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RBM10/S1-1 antibody
See all RBM10/S1-1 primary antibodies -
Description
Rabbit polyclonal to RBM10/S1-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72423 was affinity purified using an epitope specific to RBM10/S1-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A). -
Involvement in disease
Defects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs. - Information by UniProt
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Database links
- Entrez Gene: 8241 Human
- Entrez Gene: 236732 Mouse
- Omim: 300080 Human
- SwissProt: P98175 Human
- SwissProt: Q99KG3 Mouse
- Unigene: 401509 Human
- Unigene: 279194 Mouse
- Unigene: 383632 Mouse
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Alternative names
- DXS8237E antibody
- G patch domain containing protein 9 antibody
- G patch domain-containing protein 9 antibody
see all
Images
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All lanes : Anti-RBM10/S1-1 antibody (ab72423) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RBM10/S1-1 with ab72423 at 1/200 (1µg/ml). Detection: DAB.
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Detection of RBM10/S1-1 by Western Blot of Immunprecipitate.
ab72423 at 1µg/ml staining RBM10/S1-1 in HeLa whole cell lysate immunoprecipitated using ab72423 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab72423 has been referenced in 6 publications.
- Wu J et al. NPTX1 inhibits pancreatic cancer cell proliferation and migration and enhances chemotherapy sensitivity by targeting RBM10. Oncol Lett 23:154 (2022). PubMed: 35836482
- Owczarek-Lipska M et al. A TARP Syndrome Phenotype Is Associated with a Novel Splicing Variant in RBM10. Genes (Basel) 13:N/A (2022). PubMed: 36421828
- Pozzi B et al. Dengue virus targets RBM10 deregulating host cell splicing and innate immune response. Nucleic Acids Res 48:6824-6838 (2020). PubMed: 32432721
- Sun X et al. Functional role of RBM10 in lung adenocarcinoma proliferation. Int J Oncol 54:467-478 (2019). PubMed: 30483773
- Rodor J et al. The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development. RNA Biol 14:45-57 (2017). WB, CLIP ; Mouse . PubMed: 27763814
- Treiber T et al. A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Mol Cell 66:270-284.e13 (2017). PubMed: 28431233